A study on the infections caused by HPV-16, HSV-2, CMV and Chlamydia trachomatis (CT) on the uterine cervices were carried out among 188 normal cases and 544 patients with chronic cervicitis using ELISA, virus isolation and/or DNA hybridization methods. The detection rates of these four pathogens in erosive cervices were 23.9% (64/268), 26.5% (99/374), 14.0% (22/157) and 13.4% (18/134), respectively, which were significantly higher than that in normal cervices. The total detection rate of four pathogens was 48.5% in erosive cervices, 26.8% in normal cervices at fertile age and 44.1% in normal cervices during the menopausal period. Obtained results indicate a high incidence of sexually transmitted diseases in women in Beijing area. Based on above mentioned results and our experience, a simple procedure for the detection of HPV, HSV, CMV and CT on the uterine cervices was suggested.