机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA[b]Department of Biological Systems Engineering, University of Nebraska-Lincoln, Nebraska, USA[c]Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA[d]Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China临床科室血管科首都医科大学附属安贞医院[e]Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA[f]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska, USA[g]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA
Vascular smooth muscle cells (VSMCs) are of great value and are needed in large quantities for tissue engineering, drug screening, disease modeling and cell-based therapies. However, getting high quantity VSMCs remains a challenge. Here, we report a method for the scalable manufacturing of VSMCs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into VSMCs in a three dimensional (3D) thermoreversible hydrogel. The hydrogel not only acts as a 3D scaffold for cells to grow, but also protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive aggregation. Together, the hydrogel creates a cell-friendly microenvironment, leading to high culture efficiency. We show that VSMCs can be generated in 10 days with high viability (>90%), high purity (>80%) and high yield (approximate to 2.0 x 10(7) cells per mL hydrogel) in the hydrogel scaffold. The generated VSMCs have normal functions. Genome-wide gene expression analysis shows VSMCs made in the hydrogel (i.e. 3D-VSMCs) have higher expression of genes related to vasculature development and glycolysis compared to VSMCs made in the conventional 2D cultures (i.e. 2D-VSMCs), while 2D-VSMCs have higher expression of genes related to cell proliferation. This simple, defined and efficient method is scalable for manufacturing hPSC-VSMCs for various biomedical applications.
基金：
UNL-UNMC Science Engineering and Medicine (SEM) initiative grant
第一作者机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA
通讯作者：
通讯机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Nebraska, USA[b]Department of Biological Systems Engineering, University of Nebraska-Lincoln, Nebraska, USA[f]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska, USA[g]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA
推荐引用方式(GB/T 7714)：
Haishuang Lin,Qian Du,Qiang Li,et al.Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels[J].BIOMATERIALS SCIENCE.2019,7(1):347-361.doi:10.1039/c8bm01128a.
APA：
Haishuang Lin,Qian Du,Qiang Li,Ou Wang,Zhanqi Wang,...&Yuguo Lei.(2019).Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels.BIOMATERIALS SCIENCE,7,(1)
MLA：
Haishuang Lin,et al."Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels".BIOMATERIALS SCIENCE 7..1(2019):347-361
