机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska- Lincoln, Nebraska, USA.[b]Department of Biological Systems Engineering, University of Nebraska-Lincoln, Nebraska, USA[c]Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA[d]Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China临床科室血管科首都医科大学附属安贞医院[e]Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Nebraska, USA[f]Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA[g]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska, USA[h]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA
Human pluripotent stem cell derived endothelial cells (hPSC-ECs) are of great value for studying and treating vascular diseases. However, manufacturing high quantity and quality hPSC-ECs with current cell culture technologies remains very challenging. Here, we report a novel method that can manufacture hPSC-ECs in scalable and cell-friendly microenvironments to address this challenge. Using this method, hPSCs are expanded and differentiated into ECs in microscale alginate hydrogel tubes. The hydrogel tubes protect cells from the highly variable hydrodynamic conditions and critical hydrodynamic stresses in the culture vessel and limit the cell mass less than the diffusion limits (of human tissue) to ensure efficient mass transport. The hydrogel tubes provide uniform and friendly microenvironments for cells to grow. This novel design leads to extremely high production efficiency. We showed that hPSC-ECs could be produced in 10 days with high viability (>90%), high purity (>80%) and high yield (approximate to 5.0 x 10(8) cells per mL of microspace). The yield is about 250 times that of the current-state-of-the-art. hPSC-ECs made in these hydrogel tubes had similar in vitro and in vivo functions to hPSC-ECs generated by conventional cell culture methods. This simple, scalable, efficient, defined and cost-effective technology will make hPSC-ECs broadly available and affordable for various biomedical applications.
第一作者机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska- Lincoln, Nebraska, USA.
通讯作者：
通讯机构：[a]Department of Chemical and Biomolecular Engineering, University of Nebraska- Lincoln, Nebraska, USA.[c]Biomedical Engineering Program, University of Nebraska-Lincoln, Nebraska, USA[g]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska, USA[h]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA
推荐引用方式(GB/T 7714)：
Haishuang Lin,Qian Du,Qiang Li ,et al.Manufacturing human pluripotent stem cell derived endothelial cells in scalable and cell-friendly microenvironments[J].BIOMATERIALS SCIENCE.2019,7(1):373-388.doi:10.1039/c8bm01095a.
APA：
Haishuang Lin,Qian Du,Qiang Li,,Ou Wang,,Zhanqi Wang,...&Yuguo Lei.(2019).Manufacturing human pluripotent stem cell derived endothelial cells in scalable and cell-friendly microenvironments.BIOMATERIALS SCIENCE,7,(1)
MLA：
Haishuang Lin,et al."Manufacturing human pluripotent stem cell derived endothelial cells in scalable and cell-friendly microenvironments".BIOMATERIALS SCIENCE 7..1(2019):373-388
