Persistent atrial fibrillation is one of the most common arrhythmias, which is caused by disorderly excited and invalid contract room sex rhythm in patients with arrhythmia. Evidences have indicated that plasma concentration levels of bilirubin and serum uric acid are associated with progression of auricular fibrillation. In this clinical investigation, regression analysis between auricular fibrillation and plasma concentration of bilirubin and serum uric acid was analysed in patients with arrhythmia. A total of 1348 arrhythmia patients and 346 healthy volunteers were voluntarily recruited in this analysis. Cumulative incidence probability of persistent atrial fibrillation and paroxysmal atrial fibrillation were analysed. Outcomes demonstrated that plasma concentration levels of troponin, CKMB, bilirubin and serum uric acid were up-regulated in patients with arrhythmia. Our results auricular fibrillation in Left Atrium (LA) and Left Ventricle (LV) in arrhythmia patients were significantly difference with healthy volunteers. Notably, we observed that a plasma concentration level of bilirubin is positively correlated with degree of auricular fibrillation in patients with arrhythmia determined by univariate analysis. Serum uric acid is also positively correlated with degree of auricular fibrillation in patients with arrhythmia determined by univariate analysis. In conclusion, these outcomes indicate that persistent auricular fibrillation and plasma concentration levels of bilirubin and serum uric acid is up-regulated and positively correlated clinical stages in patients with arrhythmia, which may be regarded as indicator and prognostic factors for arrhythmia in clinical.