OBJECTIVE. The purpose of this article is to test the hypothesis that heart deformation analysis can automatically quantify regional myocardial motion patterns in patients with cardiac amyloidosis. SUBJECTS AND METHODS. Eleven patients with cardiac amyloidosis and 11 healthy control subjects were recruited to undergo cardiac MRI. Cine images were analyzed using heart deformation analysis and feature tracking. Heart deformation analysis-derived myocardial motion indexes in radial and circumferential directions, including radial and circumferential displacement, radial and circumferential velocity, radial and circumferential strain, and radial and circumferential strain rate, were compared between the two groups. RESULTS. The heart deformation analysis tool required a shorter mean (+/- SD) processing time than did the feature-tracking tool (1.5 +/- 0.3 vs 5.1 +/- 1.2 minutes). Patients with cardiac amyloidosis had lower peak radial displacement (4.32 +/- 1.37 vs 5.62 +/- 1.19 mm), radial velocity (25.50 +/- 7.70 vs 33.41 +/- 5.43 mm/s), radial strain (23.32% +/- 10.24% vs 31.21% +/- 8.71%), circumferential strain (-13.44% +/- 4.21% vs -17.84% +/- 2.84%), radial strain rate (1.14 +/- 0.46 vs 1.58 +/- 0.41 s(-1)), and circumferential strain rate (-0.78 +/- 0.22 vs -1.08 +/- 0.20 s(-1)) than did healthy control subjects. Heart deformation analysis-derived indexes correlated with feature tracking-derived indexes (r = 0.411 and 0.552). CONCLUSION. Heart deformation analysis is able to automatically quantify regional myocardial motion in patients with cardiac amyloidosis without the need for operator interaction.