Activation of protein kinase C epsilon enhanced movement ability and paracrine function of rat bone marrow mesenchymal stem cells partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway
Objects: to probe into the effects of PKC epsilon on migration and paracrine functions of stem cells and potential molecular mechanisms. Methods: Bone Marrow mesenchymal stem cells (BMMSCs) were obtained from rat femur and passaged. mRNA and protein levels of capital proteins in PKC epsilon signaling, SDF-1/CXCR4 axis and PI3K/AKT pathway in the MSCs in different conditions treating with PKC agonist, specific PKC epsilon inhibitor, CXCR4 antagonist or PI3K inhibitor for 24 hours were analyzed by real-time PCR and western blot, and migration abilities were observed by migration assay in vitro and the changes of paracrine factors in different treatments were analyzed by protein clips assay. Results: the levels of p-JNK, p-P38MAPK, SDF-1, CXCR4, PI3K and p-AKT increased significantly after treating with PKC agonist (P < 0.05) and decreased obviously after treating with specific PKC epsilon inhibitor. Migration ability and paracrine function of MSCs were enhanced in PMA group and attenuated in PKC epsilon inhibitor group, and inhibiting activity of CXCR4 or PI3K attenuated the effects of PKC epsilon, but not abolished completely. Conclusion: There was cross-talking between PKC epsilon signaling and SDF-1/CXCR4 axis and PI3K/AKT pathway in signal transduction of MSCs. Activating PKC epsilon could improve migration ability and paracrine function of MSCs partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway.
基金：
Beijing Anzhen Hospital, Capital University of Medical Sciences; Beijing Institute of Heart Lung and Blood Vessel Diseases; Chinese Natural Science FoundationNational Natural Science Foundation of China [81100142]; Essential Laboratory in Cardiovascular Remodeling and Transforming Medicine, National Ministry of Education [2010XGCS02]; Beijing Anzhen Hospital [2010F03]
第一作者机构：[1]Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency Cardiol, Beijing 100029, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency Cardiol, Beijing 100029, Peoples R China;[3]Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency Cardiol, Anzhen Rd Second, Beijing 100029, Peoples R China
推荐引用方式(GB/T 7714)：
He Hua,Zhao Zhi-Hong,Han Fu-Sheng,et al.Activation of protein kinase C epsilon enhanced movement ability and paracrine function of rat bone marrow mesenchymal stem cells partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE.2015,8(1):188-U1588.
APA：
He, Hua,Zhao, Zhi-Hong,Han, Fu-Sheng,Wang, Xi-Fu&Zeng, Yu-Jie.(2015).Activation of protein kinase C epsilon enhanced movement ability and paracrine function of rat bone marrow mesenchymal stem cells partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,8,(1)
MLA：
He, Hua,et al."Activation of protein kinase C epsilon enhanced movement ability and paracrine function of rat bone marrow mesenchymal stem cells partly at least independent of SDF-1/CXCR4 axis and PI3K/AKT pathway".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 8..1(2015):188-U1588
