当前位置: 首页 > 详情页

Ischemic postconditioning improves the expression of cellular membrane connexin 43 and attenuates the reperfusion injury in rat acute myocardial infarction

文献详情

资源类型:
机构: [1]Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency Cardiol, 2 Anzhen Rd, Beijing 100029, Peoples R China; [2]China Japan Friendship Hosp, Dept Cardiol, Beijing 100029, Peoples R China; [3]Pudong New Area Dist Zhoupu Hosp, Dept Cardiol, Shanghai 201318, Peoples R China
出处:
ISSN:

关键词: ischemia/reperfusion postconditioning membrane connexin 43

摘要:
To investigate the effects of cellular membrane connexin 43 (Cx43) and the potential details in ischemic postconditioning (IPOC) -induced cardioprotection, ischemia/reperfusion (IR) models were generated in 8-week-old male Sprague-Dawley rats by ligating the left coronary artery anterior descending branch. The serum levels of myocardial creatases, nitric oxide (NO) and malondialdehyde (MDA) levels, infarct size, arrhythmia events, expression and distribution of Cx43, ultrastructure and apoptosis in the myocardium in different treatments with IR, IR + IPOC, IR + diazoxide or IR + IPOC + 5-hydroxydecanoate acid (5-HD) were investigated. Consequently, IPOC decreased infarct size (10.9 vs. 43.3%, P< 0.01) and the levels of myocardial creatases, NO and MDA, and improved the expression (16.8 vs. 25.2% and 6.4 vs. 32.8%, after 1- and 3-h reperfusion, respectively; P< 0.01) and distribution of Cx43, ultrastructure and apoptosis (19.2 vs. 42.9%, P< 0.01) significantly. Diazoxide partly simulated the effects, and 5-HD attenuated but not completely abolished the effects of IPOC. In addition, the phosphorylated Cx43 (p-Cx43) level in the IR + IPOC group was lower than that in the IR + diazoxide group after 1-h reperfusion (26.1 vs. 29.4%, P> 0.05); however, it was reversed after 3-h reperfusion and the p-Cx43 level in the IR + IPOC group was significantly higher than that in the IR + diazoxide group (32.8 vs. 18.7%, P< 0.01). In conclusion, cell membrane Cx43 is also involved in the process of IPOC-induced cardioprotection and the improvement of membrane Cx43 is more dependent on mitochondrial K-ATP in the earlier phase of IPOC compared to the late phase of IPOC.

基金:
语种:
被引次数:
WOS:
PubmedID:
第一作者:
第一作者机构: [1]Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency Cardiol, 2 Anzhen Rd, Beijing 100029, Peoples R China;
通讯作者:
通讯机构: [1]Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency Cardiol, 2 Anzhen Rd, Beijing 100029, Peoples R China;
推荐引用方式(GB/T 7714):
APA:
MLA:

资源点击量:16409 今日访问量:0 总访问量:869 更新日期:2025-01-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 首都医科大学宣武医院 技术支持:重庆聚合科技有限公司 地址:北京市西城区长椿街45号宣武医院