To evaluate the accuracy, reliability and time saving potential of a novel cardiac CT (CCT)-based, automated software for the assessment of segmental left ventricular function compared to visual and manual quantitative assessment of CCT and cardiac magnetic resonance (CMR). Forty-seven patients with suspected or known coronary artery disease (CAD) were enrolled in the study. Wall thickening was calculated. Segmental LV wall motion was automatically calculated and shown as a colour-coded polar map. Processing time for each method was recorded. Mean wall thickness in both systolic and diastolic phases on polar map, CCT, and CMR was 9.2 +/- 0.1 mm and 14.9 +/- 0.2 mm, 8.9 +/- 0.1 mm and 14.5 +/- 0.1 mm, 8.3 +/- 0.1 mm and 13.6 +/- 0.1 mm, respectively. Mean wall thickening was 68.4 +/- 1.5 %, 64.8 +/- 1.4 % and 67.1 +/- 1.4 %, respectively. Agreement for the assessment of LV wall motion between CCT, CMR and polar maps was good. Bland-Altman plots and ICC indicated good agreement between CCT, CMR and automated polar maps of the diastolic and systolic segmental wall thickness and thickening. The processing time using polar map was significantly decreased compared with CCT and CMR. Automated evaluation of segmental LV function with polar maps provides similar measurements to manual CCT and CMR evaluation, albeit with substantially reduced analysis time. aEuro cent Cardiac computed tomography (CCT) can accurately assess segmental left ventricular wall function. aEuro cent A novel automated software permits accurate and fast evaluation of wall function. aEuro cent The software may improve the clinical implementation of segmental functional analysis.