Human umbilical cord blood cell transplantation improves cardiac function in a myoardial infarction rat model but induces intestinal graft versus host disease
机构：[1]Capital Med Univ, Beijing Friendship Hosp, Res Ctr Prevent Cardiovascr Dis, Beijing 100050, Peoples R China;[2]Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China;临床科室心脏外科中心首都医科大学附属安贞医院[3]China Capital Med Univ, Friendship Hosp, Res Ctr Prevent Cardiovasc Dis, Beijing 100050, Peoples R China
Human umbilical cord blood cell (HUCBC) has low immunity. In the present study we investigated intestinal graft-versus-host disease (GVHD) induced by HUCBC transplantation in a myocardial infarction (MI) rat model. MI was established by using left anterior descending coronary artery (LAD) ligation. HUCBCs were injected into the animals 5 days post MI. Four weeks after the HUCBC transplantation, histology changes in small intestine were observed under an optical microscope. In addition, cardiac functions were tested. Further, factor VIII, vascular endothelial growth factor (VEGF) in the myocardium and small intestine were assayed. The HUCBC transplantation significantly induced intestinal GVHD in the MI rats. The HUCBC implantation remarkably improved ejection fraction (EF), fractional shortening (FS), and dp/dtmax in the MI rats (P<0.05). In the myocardium, the capillary density was larger in the small intestine of the HUCBC-transplanted rats compared to the controls. Real-time PCR and western blotting revealed that VEGF mRNA and protein levels in the myocardium and the small intestine dramatically significantly upregulated in the HUCBC-transplanted rats (P<0.05). The HUCBC transplantation significantly improves aggravated cardiac function of MI rats, but it induces intestinal GVHD.
基金：
Basic and clinical research cooperation fund, capital medical university [13JK57]
第一作者机构：[1]Capital Med Univ, Beijing Friendship Hosp, Res Ctr Prevent Cardiovascr Dis, Beijing 100050, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Friendship Hosp, Res Ctr Prevent Cardiovascr Dis, Beijing 100050, Peoples R China;[3]China Capital Med Univ, Friendship Hosp, Res Ctr Prevent Cardiovasc Dis, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Xing Y.,Wu Y.,Wang L.,et al.Human umbilical cord blood cell transplantation improves cardiac function in a myoardial infarction rat model but induces intestinal graft versus host disease[J].CELLULAR AND MOLECULAR BIOLOGY.2014,60(2):6-12.doi:10.14715/cmb/2014.60.2.2.
APA：
Xing, Y.,Wu, Y.,Wang, L.&Meng, X..(2014).Human umbilical cord blood cell transplantation improves cardiac function in a myoardial infarction rat model but induces intestinal graft versus host disease.CELLULAR AND MOLECULAR BIOLOGY,60,(2)
MLA：
Xing, Y.,et al."Human umbilical cord blood cell transplantation improves cardiac function in a myoardial infarction rat model but induces intestinal graft versus host disease".CELLULAR AND MOLECULAR BIOLOGY 60..2(2014):6-12
