机构:[a]Department of Pharmacology, Xuan-wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China科技平台神经变性病教育部重点实验室首都医科大学宣武医院[b]School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
Objective: To investigate the effects of morroniside on H 2 O 2 -induced apoptosis in nerve cells. Method: Human neuroblastoma cell line SH-SY5Y cells were pre-incubaed with morroniside (1, 10, and 100 μmol·L -1 ) for 24 h prior to exposure to H 2 O 2 (500 μmol·L -1 ) for 18 h. The activity of reactive SOD was measured by a biochemical assay. The expression of caspase-3, caspase- 9, Bcl-2 and Bax was determined by Wastern blotting method. Result: Pretreatment of the cells with morroniside (10 and 100 μmol·L -1 ) increasd SOD activity by 14% (P < 0.01) and 11% (P < 0.05) in comparison with cells exposed only to H 2 O 2 . Morroniside (1, 10, 100 μmol·L -1 ) lowered caspase-3 level by 31% (P < 0.01), 103% (P < 0.001) and 95% (P < 0.001), decreased caspase-9 content by 71% (P < 0.001), 132% (P < 0.001) and 37% (P < 0.05), and increasd Bcl-1 level by 88% (P < 0.01), 121% (P < 0.001) and 60% (P < 0.01) respectively but no significant change occurred in Bax level in comparison with cells exposed only to H 2 O 2 . Conclusion: Morroniside has neuroprotection effect against H 2 O 2 -induced oxidation injury in nerve cell.
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外文
第一作者:
第一作者机构:[a]Department of Pharmacology, Xuan-wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
推荐引用方式(GB/T 7714):
Ai H.-X,Wang W,Sun F.-L,et al.Morroniside inhibits H [J].Zhongguo Zhongyao Zazhi.2008,33(18):