Aim: To investigate the effect of berberine on the proliferation of human colon cancer cell line and cyclooxygenase-2 (COX-2)/Ca 2+ pathway. Methods: 0.1, 0.3, 3.0 and 30.0 μmol · L -1 of berberine were added into the cell culture medium. MTT method was used to observe the effect of berberine on the growth and proliferation of colon cells. RT-PCR method was used to test COX-2 mRNA. Immunocytochemical method was used to test the expression of COX-2 protein. ELISA method was used to test the concentration of prostaglandin E 2 (PGE 2 ). Immunofluorescence method was used to detect [Ca 2+ ] i . Results: When the concentration of berberine was higher than 0.3 μmol · L -1 , it inhibited the growth and proliferation of colon cancer cells in a dose-dependent manner. When the concentration of berberine was higher than 0.3 μmol · L -1 , it inhibited the COX-2 mRNA and protein level. Berberine decreased the concentration of prostaglandin E 2 in the culture medium. Conclusions: Berberine can inhibit [Ca 2+ ] i and thus inhibit COX-2 mRNA level and protein expression. It can also inhibit COX-2 activity and the synthesis of PGE 2 . This may be one of the mechanisms of berberine inhibition against the growth and proliferation of HT-29 human colon cell line.
语种：
外文
第一作者：
第一作者机构：[1]首都医科大学宣武医院消化内科，北京100053
通讯作者：
通讯机构：[2]武汉大学人民医院消化内科，湖北武汉430060
推荐引用方式(GB/T 7714)：
台卫平,田 耕,黄业斌,et al.The inhibitory effect of berberine on cyclooxygenase-2/Ca [J].Chinese Pharmacological Bulletin.2005,21(8):doi:1001-1978(2005)08-0950-04.
APA：
台卫平,田 耕,黄业斌,周 俊,张泰昌&罗和生.(2005).The inhibitory effect of berberine on cyclooxygenase-2/Ca .Chinese Pharmacological Bulletin,21,(8)
MLA：
台卫平,et al."The inhibitory effect of berberine on cyclooxygenase-2/Ca ".Chinese Pharmacological Bulletin 21..8(2005)
