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Screening and characterization of human single-chain Fv antibody against β-amyloid peptide 40

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机构： [1]Department of Nuclear Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing100730 [2]GeneTherapy Research Center, Institute of Infectious Diseases,The 302 Hospital of PLA, Beijing100039 [3]Beijing Research Laboratory for Brain Aging, Xuanwu Hospital,Capital University of Medical Sciences, Beijing100053,China

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关键词： Alzheimer's disease Amyloid-β peptide Phage display Single-chain antibody

摘要：

Expression of single-chain variable fragment antibody on the surface of filamentous bacteriophage is widely used to make antibodies with pre-defined specificities. Using direct selection on solid phase-bound amyloid-β 40 peptide, wich is a main pathogenic feature of Alzheimer's disease, we obtained single-chain Fv antibody with specific binding activity. The binding epitope of the antibody is located between amino acids 1 and 16 of the peptide antigen. DNA sequencing showed that the gene coding for the single chain Fv antibody consists of 768 bp, and the complementarity-determining regions were deduced. © 2003 Lippincott Williams & Wilkins.

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最新[2025]版：

大类 | 4 区医学

小类 | 4 区神经科学

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出版当年[2001]版：

Q2 NEUROSCIENCES

最新[2024]版：

Q4 NEUROSCIENCES

影响因子： 1.7 最新[2024版] 1.5 最新五年平均 2.374 出版当年[2001版] 0 出版当年五年平均 2.696 出版前一年[2000版] 2.265 出版后一年[2002版]

第一作者：

第一作者机构： [1]Department of Nuclear Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing100730

通讯作者：

通讯机构： [1]Department of Nuclear Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing100730

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