详情页 - 首都医科大学宣武医院知识库

当前位置：首页 > 详情页

Next-generation sequencing technology as a powerful detection and semi-quantitative method for herpes simplex virus type 1 in pediatric encephalitis

文献详情

资源类型：

收录情况： ◇ SCIE

作者：

机构： [1]Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ,Dept Infect Dis,Natl Ctr Childrens Hl, Beijing, Peoples R China; [2]BGI Tianjin, Tianjin Med Lab, Tianjin, Peoples R China; [3]Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing, Peoples R China

出处：

DOI：

ISSN：

关键词： Next-generation sequencing Encephalitis Herpes simplex virus type 1 Semi-quantitative

摘要：

This case report presents a 1-year-old boy from China, with sudden onset of fever, convulsion, and sleepiness, screened for viral DNA in blood and cerebrospinal fluid (CSF) sample using next-generation sequencing (NGS) to diagnose herpes simplex virus type 1 (HSV-1) encephalitis, further validated by PCR. After acyclovir treatment, the patient's symptom disappeared and HSV-1 DNA unique reads decreased from 4290 to zero in CSF, and from 23 to zero in blood detected by NGS. The clinical presentation and outcome were consistent with the pathogenic diagnostic results of NGS. NGS of CSF samples can be used as a diagnostic assay for HSV-1 encephalitis and also might be a semi-quantitative method for evaluation of treatment effect.

基金：

语种：

被引次数：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2018]版：

大类 | 3 区医学

小类 | 3 区神经科学 3 区病毒学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区神经科学 4 区病毒学

JCR分区：

出版当年[2017]版：

Q2 NEUROSCIENCES Q2 VIROLOGY

最新[2023]版：

Q3 NEUROSCIENCES Q3 VIROLOGY

影响因子： 2.3 最新[2023版] 2.2 最新五年平均 3.228 出版当年[2017版] 3.043 出版当年五年平均 3.206 出版前一年[2016版] 2.302 出版后一年[2018版]

第一作者：

第一作者机构： [1]Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ,Dept Infect Dis,Natl Ctr Childrens Hl, Beijing, Peoples R China;

通讯作者：

通讯机构： [1]Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ,Dept Infect Dis,Natl Ctr Childrens Hl, Beijing, Peoples R China;

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Next-Generation Sequencing Technology Combined With Multiplex Polymerase Chain Reaction as a Powerful Detection and Semiquantitative Method for Herpes Simplex Virus Type 1 in Adult Encephalitis: A Case Report [2]IgG and IgM antibodies of Herpes Simplex Virus type-1 and type-2 in 233 maternal and neonatal sera [3]Doxorubicin-enriched, ALDH(br) mouse breast cancer stem cells are treatable to oncolytic herpes simplex virus type 1 [4]IgG and IgM antibodies of Herpes Simplex Virus type-1 and type-2 in 233 maternal and neonatal sera [5]单纯疱疹病毒1型感染致Mollaret脑膜炎继发癫痫发作：1例报道并文献综述 [6]Evaluation of metagenomic and pathogen-targeted next-generation sequencing for diagnosis of meningitis and encephalitis in adults: A multicenter prospective observational cohort study in China [7]二代测序在儿童线粒体病诊断中的应用 [8]Next-generation sequencing combined with routine methods to detect the pathogens of encephalitis/meningitis from a Chinese tertiary pediatric neurology center [9]Sporadic Hirschsprung Disease: Mutational Spectrum and Novel Candidate Genes Revealed by Next-generation Sequencing [10]MicroRNA expression profile in the spinal cord injured rat neurogenic bladder by next-generation sequencing