Objective: To investigate the prognostic value of dynamic susceptibility contrast-enhanced MRI (DSC-MRI), 3D-arterial spin labeling (3D-ASL) and intravoxel incoherentmotion (IVIM) in glioblastoma. Methods: From August 2016 to October 2017, a total of 34 patients with glioblastoma were enrolled. According to the overall survival (OS), they were divided into < 14 months group (17 cases) and ≥ 14 months group (17 cases). MRI conventional sequences, DSC-MRI, 3D-ASL, IVIM were acquired before operation. Multiple regions of interest (ROIs) were placed in the tumor-enhanced region and the peritumoral region, and recorded the values of DSC-maximum relative cerebral blood volume (DSC-rCBVmax) and relative cerebral blood flow (DSC-rCBF), ASL-cerebral blood flow (ASL-CBF), IVIM-perfusion fraction (PF). Receiver operating characteristic (ROC) curve was used to evaluated the prognostic value of the imaging indicators. Results: There were no significant differences in ASL-CBF, IVIM-PF, DSC-rCBF and DSC-rCBVmax between the OS < 14 months group and the OS ≥ 14 months group in tumor-enhanced region (P>0.05, for all). In the peritumoral region, the IVIM-PF of the patients in OS < 14 months was lower (P=0.011) while DSC-rCBF (P=0.009) and DSC-rCBVmax (P=0.012) were higher than OS ≥ 14 months group. The area under the ROC curve was 0.579 (95% CI:0.372-0.787, P=0.453), 0.763 (95% CI:0.588-0.937, P=0.013) and 0.746 (95% CI:0.565-0.926, P=0.020) respectively, suggesting that DSC-rCBF and DSC-rCBVmax have better reference value for prognosis. Conclusions: The imaging indicators of DSC-MRI have an effect on the OS of glioblastoma, while IVIM and ASL have not shown an effect on overall survival. Copyright © 2019 by the Editorial Board of Chinese Journal of Contemporary Neurology and Neurosurgery.