Objective:To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods:Using IL-2 and IFNγ as Th1 type cytokines,IL-4,IL-6 and IL-10 as Th2 type cytokines,the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method,and the gene expression of Th1/Th2 cytokines in human glioma cells,glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results:There was predominant expression of Th2 type cytokines in human glioma cells,glioma infiltrating lymphocytes and glioma cell lines,but there was no such expression in normal brain tissues.Conclusion:It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosuppressive status of human glioma patients.The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas.
基金：
1 Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China. Emaih [email protected]ublic.sd.cninfo.net 2 Department of Neurosurgery, Beijing XuanWu Hospital, Beijing 100053, China This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).
