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Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

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机构： [1]Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute, 518055 Shenzhen, P.R. China. [2]Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School, 518055 Shenzhen, P.R. China. [3]Open FIESTA Center, Tsinghua Shenzhen International Graduate School, 518055 Shenzhen, P.R. China. [4]Beijing Children’s Hospital, 100045 Beijing, P.R. China. [5]Additive Manufacturing Research Institute, College of Mechatronics and Control Engineering, Shenzhen University, 518060 Shenzhen, P.R. China. [6]Tongji Medical College, Huazhong University Science & Technology, 430022 Wuhan, P.R. China. [7]Shaanxi Institute of Ophthalmology, 710002 Xi’an, P.R. China. [8]Shaanxi Key Laboratory of Eye, 710002 Xi’an, P.R. China. [9]Department of Mechanical Engineering, Tsinghua University, 100084 Beijing, P.R. China. [10]Department of Mechanical Engineering and Mechanics, Drexel University, 19104 Philadelphia, PA, USA

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Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma. © 2020, The Author(s).

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出版当年[2019]版：

大类 | 1 区

综合性期刊

小类 | 1 区综合性期刊

最新[2023]版：

大类 | 1 区

综合性期刊

小类 | 1 区综合性期刊

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出版当年[2018]版：

Q1 MULTIDISCIPLINARY SCIENCES

最新[2023]版：

Q1 MULTIDISCIPLINARY SCIENCES

影响因子： 14.7 最新[2023版] 16.1 最新五年平均 11.878 出版当年[2018版] 13.811 出版当年五年平均 12.353 出版前一年[2017版] 12.121 出版后一年[2019版]

第一作者：

第一作者机构： [1]Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute, 518055 Shenzhen, P.R. China. [2]Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School, 518055 Shenzhen, P.R. China.

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通讯作者：

通讯机构： [1]Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute, 518055 Shenzhen, P.R. China. [2]Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School, 518055 Shenzhen, P.R. China. [3]Open FIESTA Center, Tsinghua Shenzhen International Graduate School, 518055 Shenzhen, P.R. China. [9]Department of Mechanical Engineering, Tsinghua University, 100084 Beijing, P.R. China. [10]Department of Mechanical Engineering and Mechanics, Drexel University, 19104 Philadelphia, PA, USA

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