机构：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China[2]Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China首都医科大学附属北京友谊医院[3]Key Laboratory of Uighur Medicine of Xinjiang Uygur Autonomous Region, Xinjiang Institute of Materia Medica, Urumqi 830004, China[4]Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属天坛医院[5]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China神经科系统神经内科首都医科大学宣武医院
MicroRNAs (miRNAs) are small noncoding RNAs, which regulate numerous cell functions by targeting mRNA for cleavage or translational repression, and have been found to play an important role in Alzheimer's disease (AD). Our study aimed to identify differentially expressed miRNAs in AD brain as a reference of potential therapeutic miRNAs or biomarkers for this disease. We used amyloid precursor protein (APP) and presenilin 1 (PS1) double transgenic mice and age-matched wild-type (WT) littermates to determine the expression of miRNAs in the brain. MiRNAs were profiled by microarray, and differentially expressed miRNAs underwent target prediction and enrichment analysis. Microarray analysis revealed 56 differentially expressed miRNAs in AD mouse brain, which involved 39 miRNAs that were significantly upregulated and 19 that were downregulated at different ages. Among those miRNAs, a total of 11 miRNAs, including miR-342-3p, miR-342-5p, miR-376c-3p, and miR-301b-3p, were not only conserved in human but also predicted to have targets and signaling pathways closely related to the pathology of AD. In conclusion, in this study, differentially expressed miRNAs were identified in AD brain and proposed as biomarkers, which may have the potential to indicate AD progression. Despite being preliminary, these results may aid in investigating pathological hallmarks and identify effective therapeutic targets.
基金：
National Natural Science
Foundation of China (nos. 81673411 and 81670474), Key Laboratory
of Uighur Medicine Foundation of Xinjiang Uygur
Autonomous Region (2015KL005), and CAMS Innovation
Fund for Medical Science (2017-I2M-1-016).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2023]版：
无
JCR分区：
出版当年[2015]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
通讯作者：
推荐引用方式(GB/T 7714)：
Lin-lin Wang,Li Min,Qing-dong Guo,et al.Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease[J].BIOMED RESEARCH INTERNATIONAL.2017,2017:doi:10.1155/2017/8030369.
APA：
Lin-lin Wang,Li Min,Qing-dong Guo,Jun-xia Zhang,Hai-lun Jiang...&Shui-long Guo.(2017).Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease.BIOMED RESEARCH INTERNATIONAL,2017,
MLA：
Lin-lin Wang,et al."Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease".BIOMED RESEARCH INTERNATIONAL 2017.(2017)
