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Assays to Examine Endothelial Cell Migration, Tube Formation, and Gene Expression Profiles

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机构： [1]Neuroprotection Research Laboratory, Department of Radiology and Neurology Harvard Medical School, Massachusetts General Hospital Boston USA [2]Department of PediatricsHarvard Medical School, Massachusetts General Hospital Boston USA [3]Cerebrovascular Research Center, Xuanwu HospitalCapital Medical University Beijing China

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关键词： Angiogenesis Endothelial cells Matrigel matrix Tube formation Migration Transcriptome Microparticles

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Common methods for studying angiogenesis in vitro include the tube formation assay, the migration assay, and the study of the endothelial genome. The formation of capillary-like tubes in vitro on basement membrane matrix mimics many steps of the angiogenesis process in vivo and is used widely as a screening test for angiogenic or antiangiogenic factors. Other assays related to the study of angiogenesis include the cell migration assay, the study of gene expression changes during the process of angiogenesis, and the study of endothelial-derived microparticles. Protocols for these procedures will be described here.

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第一作者机构： [1]Neuroprotection Research Laboratory, Department of Radiology and Neurology Harvard Medical School, Massachusetts General Hospital Boston USA

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