机构：[1]Department of Neurology and Institute of Neurology, First Affiliated Hospital, Institute of Neuroscience, Fujian Medical University, Fuzhou, China[2]Department of Neurology, Tianjin Medical University General Hospital, Tianjin, China[3]Department of Neurology, Xuanwu Hospital, Capital MedicalUniversity, Beijing, China神经科系统神经内科首都医科大学宣武医院[4]Department of Function Neuroimaging, Neurosurgical Institute, Capital Medical University, Beijing, China[5]Key Laboratory ofthe Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an, China
Background Noninvasive evaluation of the status of cerebral arteriole perfusion remains a practical challenge in murine stroke models, because conventional magnetic resonance imaging (MRI) is no longer capable of capturing these very small vessels. Purpose To investigate the feasibility of ultrasmall superparamagnetic iron oxide particles (USPIO)-based susceptibility weighted imaging (SWI)-MRI (USPIO-SWI) and T2* map-MRI (USPIO-T2* map) for monitoring angiographic perfusion in stroke rats. Study Type A preclinical randomized controlled trial. Animal Model Normal rats (N = 9), embolic middle cerebral artery occlusion (eMCAO) rats (N = 66). Field Strength/Sequence 7 T; T2* map (multigradient echo), SWI (3D gradient echo). Assessment Experiment 1: To develop a method for angiographic reperfusion evaluation with USPIO-SWI. Normal rats were used to optimize the USPIO dosage (5.6, 16.8, and 56 mg/kg ferumoxytol) as well as scan time points for cerebral arterioles. Contrast-to-noise ratio (CNR) was measured. Stroke rats were further used and the number of visual cortical vessels were counted. Experiment 2: To examine whether fingolimod (lymphocytes inhibitor) enhances the action of tissue plasminogen activator (tPA) in eMCAO rats on cerebral angiographic reperfusion. Statistical Tests Mann-Whitney test and two way-ANOVA were used. P < 0.05 was considered statistically significant. Results CNR values of cerebral cortical penetrating arteries in normal rats were significantly increased to 4.4 +/- 0.5 (5.6 mg/kg), 6.1 +/- 0.5 (16.8 mg/kg), and 3.4 +/- 0.9 (56 mg/kg) after USPIO injection. The number of visual cortical vessels on USPIO-SWI images in ischemic regions was significantly less than in control regions (5 +/- 2 vs. 56 +/- 20) of eMCAO rats. Compared with eMCAO rats who received tPA only, eMCAO rats who received the combination of fingolimod and tPA exhibited significantly higher proportion of complete angiographic reperfusion (69% vs. 17%). Data Conclusion This study supports the feasibility of angiographic perfusion evaluation with USPIO-SWI in stroke rats. Level of Evidence 2 Technical Efficacy Stage 1
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81771279]
第一作者机构：[1]Department of Neurology and Institute of Neurology, First Affiliated Hospital, Institute of Neuroscience, Fujian Medical University, Fuzhou, China
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推荐引用方式(GB/T 7714)：
Fu Ying,Zhao Wenlong,Lin Kunxin,et al.USPIO-SWI Shows Fingolimod Enhanced Alteplase Action on Angiographic Reperfusion in eMCAO Rats[J].JOURNAL OF MAGNETIC RESONANCE IMAGING.2022,55(4):1095-1106.doi:10.1002/jmri.27914.
APA：
Fu, Ying,Zhao, Wenlong,Lin, Kunxin,Lv, Aowei,Tian, Lili...&Yan, Yaping.(2022).USPIO-SWI Shows Fingolimod Enhanced Alteplase Action on Angiographic Reperfusion in eMCAO Rats.JOURNAL OF MAGNETIC RESONANCE IMAGING,55,(4)
MLA：
Fu, Ying,et al."USPIO-SWI Shows Fingolimod Enhanced Alteplase Action on Angiographic Reperfusion in eMCAO Rats".JOURNAL OF MAGNETIC RESONANCE IMAGING 55..4(2022):1095-1106
