Induced neural stem cells from Macaca fascicularis show potential of dopaminergic neuron specification and efficacy in a mouse Parkinson?s disease model
机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China内科系统科技平台老年医学科神经变性病教育部重点实验室首都医科大学宣武医院[2]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[3]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China[4]College of Life Sciences, Jiaying University, Meizhou, Guangdong Province 514015, China
Induced neural stem cells (iNSCs) can be reprogrammed from somatic cells and have shown potentials in treatment of various neurological diseases/disorders. Obtaining iNSCs of nonhuman primates serves as an important bridge for clinical translation using iNSCs. In the current study, cynomolgus (Macaca fascicularis) bone marrow mesenchymal stromal cells (MSCs) were reprogrammed into iNSCs by transduction of nonintegrative Sendai virus encoding transgenes OCT4, SOX2, KLF4 and C-MYC. The obtained iNSCs showed characteristics of normal neural stem cells (NSCs) and could differentiate into neurons, astrocytes and oligodendrocytes. Furthermore, iNSCs could give rise to dopaminergic neural cells in vitro, which showed safety and efficacy after transplantation into the striatum of an immunodeficient mouse Parkinson's disease (PD) model.
基金：
Stem Cell and Translation National Key Project [2016YFA0101403]; National Natural Science Foundation of China [82171250, 81973351]; Beijing Municipal Natural Science Foundation [5142005]; Beijing Talents Foundation [2017000021223TD03]; Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [CIT TCD20180333]; Beijing Medical System High Level Talent Award [2015-3-063]; Beijing Municipal Health Commission Fund [PXM2020_026283_000005]; Beijing One Hundred, Thousand, and Ten Thousand Talents Fund [2018A03]; Royal Society-Newton Advanced Fellowship [NA150482]
第一作者机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China[2]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[3]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China
通讯作者：
通讯机构：[1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China[2]Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100069, China[3]Center of Parkinson’s Disease, Beijing Institute for Brain Disorders, Beijing 100069, China[*1]Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Li Fengyan,Zhang Aixia,Li Mo,et al.Induced neural stem cells from Macaca fascicularis show potential of dopaminergic neuron specification and efficacy in a mouse Parkinson?s disease model[J].ACTA HISTOCHEMICA.2022,124(6):doi:10.1016/j.acthis.2022.151927.
APA：
Li, Fengyan,Zhang, Aixia,Li, Mo,Wang, Xueyao,Wang, Xuan...&Chen, Zhiguo.(2022).Induced neural stem cells from Macaca fascicularis show potential of dopaminergic neuron specification and efficacy in a mouse Parkinson?s disease model.ACTA HISTOCHEMICA,124,(6)
MLA：
Li, Fengyan,et al."Induced neural stem cells from Macaca fascicularis show potential of dopaminergic neuron specification and efficacy in a mouse Parkinson?s disease model".ACTA HISTOCHEMICA 124..6(2022)
