This study investigates the effectiveness of repetitive transcranial magnetic stimulation (rTMS) as a nonpharmacological approach to treating neuropathic pain (NP), a major challenge in clinical research. Conducted on male Sprague-Dawley rats with NP induced through chronic constriction injury of the sciatic nerve, the research assessed pain behaviors and the impact of rTMS on molecular interactions within the amygdala. Through a comprehensive analysis involving Mechanical Withdrawal Threshold (MWT), Thermal Withdrawal Latency (TWL), RNA transcriptome sequencing, RT-qPCR, Western blotting, immunofluorescence staining, and Co-Immunoprecipitation (Co-IP), the study focused on the expression and interaction of integrin alpha v beta 3 and its receptor P2X7R. Findings reveal that rTMS significantly influences the expression of integrin alpha v beta 3 in NP models, suggesting an inhibition of the NP-associated NLRP3 inflammatory pathway through the disruption of integrin alpha v beta 3-P2X7R interactions. These outcomes highlight the potential of rTMS in alleviating NP by targeting molecular interactions within the amygdala, offering a promising therapeutic avenue for managing NP.
基金：
This work was supported by Natural Science Foundation of
China (82360164); Project of Hunan Provincial Department of Education (23A0729); Health Research Project of Hunan Provincial Health
Commission (202218015847, 20243295) and Natural Science Foundation of Hunan Province (2023SK4014).
第一作者机构：[1]Hunan Univ Med, Peoples Hosp Huaihua 1, Gen Hosp, Dept Anesthesiol, 144 South Jinxi Rd, Huaihua 418000, Hunan, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Hunan Univ Med, Peoples Hosp Huaihua 1, Gen Hosp, Dept Anesthesiol, 144 South Jinxi Rd, Huaihua 418000, Hunan, Peoples R China[2]Univ South China, Affiliated Hosp 1, Dept Anesthesiol, Hengyang 421001, Peoples R China[4]Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21287 USA[5]Capital Med Univ, Xuanwu Hosp, Dept Pain Management, 45 Changchun St, Beijing 100053, Peoples R China[6]Cedars Sinai Med Ctr, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
推荐引用方式(GB/T 7714)：
Zhang Zhenhua,Hou Zixin,Han Mingming,et al.Amygdala-Targeted Relief of Neuropathic Pain: Efficacy of Repetitive Transcranial Magnetic Stimulation in NLRP3 Pathway Suppression[J].MOLECULAR NEUROBIOLOGY.2024,61(11):8904-8920.doi:10.1007/s12035-024-04087-7.
APA：
Zhang, Zhenhua,Hou, Zixin,Han, Mingming,Guo, Peng,Chen, Kemin...&Yang, Fengrui.(2024).Amygdala-Targeted Relief of Neuropathic Pain: Efficacy of Repetitive Transcranial Magnetic Stimulation in NLRP3 Pathway Suppression.MOLECULAR NEUROBIOLOGY,61,(11)
MLA：
Zhang, Zhenhua,et al."Amygdala-Targeted Relief of Neuropathic Pain: Efficacy of Repetitive Transcranial Magnetic Stimulation in NLRP3 Pathway Suppression".MOLECULAR NEUROBIOLOGY 61..11(2024):8904-8920
