个人科研网厅 | | 帮助
首都医科大学宣武医院知识库
高级检索
当前位置： 首页 > 详情页

Construction of ceRNA network mediated by circRNAs screening from microarray and identification of novel biomarkers for myasthenia gravis

| 认领 | 导出 | |

文献详情

资源类型：
WOS体系：
Pubmed体系：

收录情况： ◇ SCIE

作者：
Kong Xiaotong[1] * ; Wu Tao[2] * ; Cai Hanlu[1] ; Chen Zhimin[1,3] ; Wang Yu[1] ; He Ping[4] ; Liu Peifang[1] ; Li Lei[1] ; Peng Shanshan[1] ; Xu Fanfan[1] ; Wang Jianjian[1] ; Zhang Huixue[1] ; Wang Lihua[1] ;
机构： [1]Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China [2]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China [3]Department of Neurology, The Second Hospital of Harbin, Harbin, Heilongjiang Province, China [4]Department of Neurology, The First Hospital of Harbin, Harbin, Heilongjiang Province, China
出处：
DOI： 10.1016/j.gene.2024.148463
ISSN：

关键词： CircRNA ceRNA Myasthenia gravis Biomarker

摘要：
Recent studies have revealed that circRNA can serve as ceRNA to participate in multiple autoimmune diseases. Our study aims to explore the key circRNA as ceRNA and biomarker for MG.We used circRNA microarray to explore differentially expressed circRNAs (DECs) from MG and compare with control. Then, we predicted the target miRNA associated with DECs and screened miRNAs by the algorithm of random walk with restart (RWR). Next, we constructed the circRNA-miRNA-mRNA ceRNA regulated network (CMMC) to identify the hub objects. Following, we detected the expression of hub-circRNAs by RT-PCR. We verify has_circ_0004183 (circFRMD4) sponging miR-145-5p regulate cells proliferation using luciferase assay and CCK-8.We found that the expression level of circFRMD4 and has_circ_0035381 (circPIGB) were upregulated and has_circ_0089153(circ NUP214) had the lowest expression level in MG. Finally, we proved circFRMD4 sponging miR-145-5p regulate Jurkat cells proliferation. CircFRMD4 take part in the genesis and development of MG via circFRMD4/miR145-5p axis.We found that circFRMD4, circPIGB and circNUP214 can be considered as valuable potential novel biomarkers for AchR + MG. CircFRMD4 participate in the development of AchR + MG via targeting binding with miR-145-5p.Copyright © 2024 Elsevier B.V. All rights reserved.

基金：
This study was supported by the National Natural Science Foundation of China (grant nos. 81820108014, 82171396 and 82071407). The Postdoctoral Foundation of Heilongjiang Province (grant nos. LBH-Z19181), China Postdoctoral Science Foundation Grant (grant nos. 2020M681114) and Young Elite Scientist Sponsorship Program by Heilongjiang Province (grant nos. 2022QNTJ003).
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类 | 3 区 生物学
小类 | 3 区 遗传学
最新[2023]版：
大类 | 3 区 生物学
小类 | 3 区 遗传学
JCR分区：
出版当年[2022]版：
Q2 GENETICS & HEREDITY
最新[2023]版：
Q2 GENETICS & HEREDITY

影响因子： 最新[2023版] 最新五年平均 出版当年[2022版] 出版当年五年平均 出版前一年[2021版] 出版后一年[2023版]

第一作者：
第一作者机构： [1]Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Kong Xiaotong,Wu Tao,Cai Hanlu,et al.Construction of ceRNA network mediated by circRNAs screening from microarray and identification of novel biomarkers for myasthenia gravis[J].GENE.2024,918:148463.doi:10.1016/j.gene.2024.148463.
APA：
Kong Xiaotong,Wu Tao,Cai Hanlu,Chen Zhimin,Wang Yu...&Wang Lihua.(2024).Construction of ceRNA network mediated by circRNAs screening from microarray and identification of novel biomarkers for myasthenia gravis.GENE,918,
MLA：
Kong Xiaotong,et al."Construction of ceRNA network mediated by circRNAs screening from microarray and identification of novel biomarkers for myasthenia gravis".GENE 918.(2024):148463

相关文献

[1]LncRNA LINC00680 Acts as a Competing Endogenous RNA and Is Associated With the Severity of Myasthennia Gravis [2]Phenotypes of myasthenia gravis, volume II [3]Editorial: Phenotypes of myasthenia gravis [4]Myasthenia Gravis Registry in China [5]Autoantibody levels as biomarkers of disease activity in management of myasthenia gravis [6]Study of Telitacicept in Generalized Myasthenia Gravis [7]重症肌无力误诊一例 [8]婴儿期起病的自身免疫性重症肌无力临床研究 [9]Myasthenia gravis-associated thymoma and myasthenia gravis-free thymoma have distinct somatic mutation and gene expression profiles. [10]扩大胸腺切除治疗重症肌无力的围手术期管理

资源点击量：16409 今日访问量：0 总访问量：869 更新日期：2025-01-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 首都医科大学宣武医院 技术支持：重庆聚合科技有限公司 地址：北京市西城区长椿街45号宣武医院