Accurate diagnosis is vital for treating primary central nervous system lymphoma (PCNSL) and determining patient prognosis in clinical practice. Currently, histological analysis stands as the gold standard for definitively diagnosing PCNSL, yet it is time-consuming and invasive. This study introduces multiphoton microscopy (MPM), utilizing second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), to detect human PCNSL. Several diagnostic features of PCNSL, such as increased cellularity, angiocentric infiltration pattern, geographic necrosis, perivascular reticulin deposits, and apoptosis niche, are captured. Moreover, with image processing, the extent of necrosis and perivascular reticulin deposits can be automatically assessed. These research findings underscore the capability of MPM for PCNSL identification. With the advancements in multiphoton endoscopes, in vivo detection of PCNSL may be achievable.
基金：
Scientific Research Project of the National Key Clinical Specialty Construction Project; [2022YBL-ZD-03]
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2025]版：
大类|3 区物理与天体物理
小类|3 区生物物理4 区生化研究方法4 区光学
最新[2025]版：
大类|3 区物理与天体物理
小类|3 区生物物理4 区生化研究方法4 区光学
JCR分区：
出版当年[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3BIOPHYSICSQ3OPTICS
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3BIOPHYSICSQ3OPTICS
第一作者机构：[1]Capital Med Univ, Xuanwu Hosp, Dept Pathol, Beijing, Peoples R China[2]Fujian Med Univ, Affiliated Hosp 1, Dept Pathol, Fuzhou, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Xingfu,Fang Na,Hu Liwen,et al.Rapid, Label-Free Detection of Primary Central Nervous System Lymphoma Using Multiphoton Microscopy[J].JOURNAL OF BIOPHOTONICS.2025,doi:10.1002/jbio.70014.
APA：
Wang, Xingfu,Fang, Na,Hu, Liwen,Wu, Zanyi,Li, Lianhuang...&Piao, Yueshan.(2025).Rapid, Label-Free Detection of Primary Central Nervous System Lymphoma Using Multiphoton Microscopy.JOURNAL OF BIOPHOTONICS,,
MLA：
Wang, Xingfu,et al."Rapid, Label-Free Detection of Primary Central Nervous System Lymphoma Using Multiphoton Microscopy".JOURNAL OF BIOPHOTONICS .(2025)
