Downregulation of long non-coding RNA UCA1 enhances the radiosensitivity and inhibits migration via suppression of epithelial-mesenchymal transition in colorectal cancer cells
机构：[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Department of General Surgery, The First People's Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, Taicang, Jiangsu 215400[3]School of Radiation Medicine and Protection, Medical College of Soochow University[4]Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, Jiangsu 215123[5]Department of General Surgery, The Laiwu City People's Hospital, Laiwu, Shandong 271100[6]Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
Colorectal cancer (CRC) is the third most commonly diagnosed cancer and common cause of cancer-related deaths. Radiotherapy has become a routine treatment for CRC. However, radioresistance affects therapeutic efficacy. Long non-coding RNA urothelial carcinoma associated 1 (UCA1) has been demonstrated to be overexpressed in several tumors and predicts a poor prognosis. In the present study, we revealed that lncRNA-UCA1 was overexpressed in colorectal cancer tissue and colon cancer cells when compared to normal tissue and cells. Quantitative real-time PCR revealed that the expression of UCA1 was significantly higher in CRC tissues after chemoradiotherapy. Downregulation of UCA1 enhanced the radiosensitivity of CCL244 cells via inhibition of the colony formation, proliferation and promotion of radiation-induced apoptosis and G2/M arrest. Moreover, downregulation of UCA1 suppressed the epithelial-mesenchymal transition (EMT) in CCL244 cells.
基金：
The present study was partially supported by the National
Natural Science Foundation of China (grant nos. 81672970,
81301933, 81472917 and 81572345), the Natural Science
Foundation of Jiangsu Province (no. BK20160338), the projects
of Suzhou Technology Bureau (nos. SZS201618, SYS201552
and SYSD2015034), the Focus of Clinical Disease Treatment
Technology Special Funds of Suzhou City (LCZX201505)
and the Second Affiliated Hospital of Soochow University
Preponderant Clinic Discipline Group Project Funding
第一作者机构：[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004
共同第一作者：
通讯作者：
通讯机构：[*1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu 215004, P.R. China
推荐引用方式(GB/T 7714)：
XIAODONG YANG,WEI LIU,XIAOHUI XU,et al.Downregulation of long non-coding RNA UCA1 enhances the radiosensitivity and inhibits migration via suppression of epithelial-mesenchymal transition in colorectal cancer cells[J].ONCOLOGY REPORTS.2018,40(3):1554-1564.doi:10.3892/or.2018.6573.
APA：
XIAODONG YANG,WEI LIU,XIAOHUI XU,JUNJIA ZHU,YONG WU...&CHUNGEN XING.(2018).Downregulation of long non-coding RNA UCA1 enhances the radiosensitivity and inhibits migration via suppression of epithelial-mesenchymal transition in colorectal cancer cells.ONCOLOGY REPORTS,40,(3)
MLA：
XIAODONG YANG,et al."Downregulation of long non-coding RNA UCA1 enhances the radiosensitivity and inhibits migration via suppression of epithelial-mesenchymal transition in colorectal cancer cells".ONCOLOGY REPORTS 40..3(2018):1554-1564
