机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China[2]Department of Oncology, The Second People’s Hospital of Taizhou, Taizhou, Jiangsu Province, P.R. China[3]Department of Oncology, Affiliated Hospital of Jiangnan University, The Fourth People’s Hospital of Wuxi, Wuxi, Jiangsu, P.R. China
We aimed to investigate the significant role of long noncoding RNA X inactive specific transcript (XIST) in regulating tumor metastasis in colorectal cancer (CRC), as well as its possible mechanism. Expression of lncRNA XIST in CRC tissues and CRC cells was detected. CRC cells were transfected with pc-XIST, blank control si-XIST, or si-control, and then the effects of lncRNA XIST on CRC cell migration and invasion were investigated, along with the interaction between lncRNA XIST and miR-137. lncRNA XIST was upregulated in CRC tissues. Compared with HT29 cells that had low metastatic potential, XIST was markedly more highly expressed in LoVo cells that had a higher metastatic potential. Overexpression of XIST promoted the migratory and invasive potential of HT29 cells, while knockdown of XIST inhibited the migratory and invasive potential of LoVo cells. Moreover, epithelial-mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, and vimentin, exhibited corresponding expression changes. In addition, miR-137 was inhibited by XIST, and inhibition of miR-137 could reverse the effects of knockdown of XIST on the migratory and invasive potential of LoVo cells. Furthermore, enhancer of zeste homolog 2 (EZH2) was confirmed as a target of miR-137. Our data reveal that lncRNA XIST may promote tumor metastasis in CRC possibly through regulating the miR-137-EZH2 axis. lncRNA XIST may serve as a prognostic indicator for CRC progression.
第一作者机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China[2]Department of Oncology, The Second People’s Hospital of Taizhou, Taizhou, Jiangsu Province, P.R. China
通讯作者：
通讯机构：[*1]Department of Oncology, The Second Affiliated Hospital of Soochow University, NO 1055 Sanxiang Road, Suzhou, Jiangsu 215004, P.R. China
推荐引用方式(GB/T 7714)：
Xingxiang Liu,Lin Cui,Dong Hua.Long Noncoding RNA XIST Regulates miR-137-EZH2 Axis to Promote Tumor Metastasis in Colorectal Cancer[J].ONCOLOGY RESEARCH.2018,27(1):99-106.doi:10.3727/096504018X15195193936573.
APA：
Xingxiang Liu,Lin Cui&Dong Hua.(2018).Long Noncoding RNA XIST Regulates miR-137-EZH2 Axis to Promote Tumor Metastasis in Colorectal Cancer.ONCOLOGY RESEARCH,27,(1)
MLA：
Xingxiang Liu,et al."Long Noncoding RNA XIST Regulates miR-137-EZH2 Axis to Promote Tumor Metastasis in Colorectal Cancer".ONCOLOGY RESEARCH 27..1(2018):99-106
