机构：[1]Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China[2]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China[3]Center for Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsum, China[4]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
Exosomes are critically involved in tumor growth, metastasis, and therapy resistance. Exosomes have the potential to be utilized as cancer biomarkers. In this study, we aimed to explore the roles and clinical values of exosomal miRNAs in gastric cancer. We found that the concentration of exosomes was significantly higher in the serum of gastric cancer patients and the culture supernatants of gastric cancer cells than that in healthy volunteers and gastric mucosa epithelial cells. In particular, miR-423-5p was elevated in the serum exosomes of gastric cancer patients, and the level of exosomal miR-423-5p was remarkably correlated with lymph node metastasis. High level of exosomal miR-423-5p was associated with poor outcome in gastric cancer patients. MiR-423-5p enriched exosomes could be internalized into gastric cancer cells, which enhanced cell proliferation and migration both in vitro and in vivo. Mechanistically, miR-423-5p inhibited the expression of sup pressor f fused protein (SUFU) to enhance the proliferation and migration of gastric cancer cells. The expression levels of SUFU were significantly decreased in gastric cancer cells and the tumor tissues of gastric cancer patients. Taken together, our findings indicate that exosomes could deliver miR-423-5p to promote cancer growth and metastasis and serum exosomal miR-423-5p may s-erve as a potential marker for gastric cancer diagnosis and prognosis.
基金：
National Natural Science Foundation of China,
Grant numbers: 81572075, 81702078;
Jiangsu Key Research and Development
Project, Grant numbers: BE2015667,
BE2016717; Postdoctoral Science Foundation
of China, Grant number: 2016M591791;
Priority Academic Program Development of
Jiangsu Higher Education Institutions; Natural
Science Foundation of Jiangsu Province,
Grant number: BK20170356; Natural Science
Fund for Colleges and Universities of Jiangsu
Province, Grant number: 17KJB320016;
Suzhou Science and Technology Project,
Grant number: SYS201728
第一作者机构：[1]Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China[2]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China
通讯作者：
通讯机构：[*1]Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China
推荐引用方式(GB/T 7714)：
Huan Yang,Hailong Fu,Bo Wang,et al.Exosomal miR-423-5p targets SUFU to promote cancer growth and metastasis and serves as a novel marker for gastric cancer[J].MOLECULAR CARCINOGENESIS.2018,57(9):1223-1236.doi:10.1002/mc.22838.
APA：
Huan Yang,Hailong Fu,Bo Wang,Xu Zhang,Jiahui Mao...&Wenrong Xu.(2018).Exosomal miR-423-5p targets SUFU to promote cancer growth and metastasis and serves as a novel marker for gastric cancer.MOLECULAR CARCINOGENESIS,57,(9)
MLA：
Huan Yang,et al."Exosomal miR-423-5p targets SUFU to promote cancer growth and metastasis and serves as a novel marker for gastric cancer".MOLECULAR CARCINOGENESIS 57..9(2018):1223-1236
