机构：[1]Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People’s Republic of China[2]Biomanufacturing Engineering Research Laboratory, Graduate School at Shenzhen Tsinghua University, Shenzhen 518055, People’s Republic of China[3]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, People’s Republic of China[4]Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, People’s Republic of China[5]Medprin Biotech GmbH, Gutleutstr 163?167, 60327 Frankfurt, Germany[6]Department of Stomatology, Peking Union Medical College Hospital, CAMS and PUMC, Beijing 100730, People’s Republic of China[7]Department of Precision Medicine and Healthcare, Tsinghua Berkeley Shenzhen Institute, Shenzhen 518055, People’s Republic of China
Three-dimensional (3D) bioprinting composite alginate-gelatin hydrogel has encouraged the fabrication of cell-laden functional structures with cells from various tissues. However, reports focusing on printing this hydrogel for nerve tissue research are limited. This study aims at building in vitro Schwann cell 3D microenvironment with customized shapes through 3D bioprinting technology. Rat Schwann cell RSC96s encapsulated in composite alginate-gelatin hydrogel were printed with an extrusion-based bioprinter. Cells maintained high viability of 85.35 +/- 6.19% immediately after printing and the printed hydrogel supported long-term Schwann cell proliferation for 2 weeks. Furthermore, after 14 days of culturing, Schwann cells cultured in printed structures maintained viability of 92.34 +/- 2.19% and showed enhanced capability of nerve growth factor (NGF) release (142.41 +/- 8.99 pg/ml) compared with cells from two-dimensional culture (92.27 +/- 9.30 pg/ml). Specific Schwann cell marker S100 beta was also expressed by cells in printed structures. These printed structures may have the potential to be used as in vitro neurotrophic factor carriers and could be integrated into complex biomimetic artificial structures with the assistance of 3D bioprinting technology.
基金：
This work is partly supported by the following
programs: Chinese army open Grant (No. BWS17J036); China Shenzhen
Peacock Plan Project (No. KQTD201209); and ‘Biomanufacturing
and Engineering Living Systems’ Overseas Expertise Introduction
Center for Discipline Innovation (No. G2017002).
第一作者机构：[1]Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People’s Republic of China
通讯作者：
通讯机构：[1]Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People’s Republic of China[2]Biomanufacturing Engineering Research Laboratory, Graduate School at Shenzhen Tsinghua University, Shenzhen 518055, People’s Republic of China[6]Department of Stomatology, Peking Union Medical College Hospital, CAMS and PUMC, Beijing 100730, People’s Republic of China[7]Department of Precision Medicine and Healthcare, Tsinghua Berkeley Shenzhen Institute, Shenzhen 518055, People’s Republic of China
推荐引用方式(GB/T 7714)：
Li Xinda,Wang Xiong,Wang Xuanzhi,et al.3D bioprinted rat Schwann cell-laden structures with shape flexibility and enhanced nerve growth factor expression[J].3 BIOTECH.2018,8(8):342.doi:10.1007/s13205-018-1341-9.
APA：
Li, Xinda,Wang, Xiong,Wang, Xuanzhi,Chen, Hongqing,Zhang, Xinzhi...&Xu, Tao.(2018).3D bioprinted rat Schwann cell-laden structures with shape flexibility and enhanced nerve growth factor expression.3 BIOTECH,8,(8)
MLA：
Li, Xinda,et al."3D bioprinted rat Schwann cell-laden structures with shape flexibility and enhanced nerve growth factor expression".3 BIOTECH 8..8(2018):342
