Static Compression Induces ECM Remodeling and Integrin alpha 2 beta 1 Expression and Signaling in a Rat Tail Caudal Intervertebral Disc Degeneration Model
机构：[1]The Second Affiliated Hospital of Soochow University, Suzhou, China[2]State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Study Design. A three-level rat tail caudal intervertebral disc (IVD) degeneration (IVDD) model was established to study effects of static compression on extracellular matrix (ECM) remodeling and integrin signaling in IVDs during IVDD. Objective. The aim of this study was to investigate the effect of compression force on ECM remodeling and integrin signaling in IVDs during IVDD. Summary of Background Data. Integrins sense mechanical environment alteration via binding to ECM ligands and trigger intracellular signaling for pathological ECM remodeling during IVDD. However, the role of compression force in ECM remodeling and integrin signaling during IVDD remains elusive. Methods. Compared with the classical one-level rat tail IVDD model that exerts axial stress on the 8th to 9th caudal vertebral bodies, a three-level model was established by using an Ilizarovtype apparatus to exert stress on the 7th to 10th caudal vertebral bodies in rat tails for four weeks. To exclude side effects from surgical stab injury on manipulated discs, intact coccygeal (Co) disc Co8-9 was analyzed. Results. In three-level IVDD model, significant degeneration of the Co8-9 disc was observed. Quantitative real-time polymerase chain reaction (qRT-PCR) showed elevated mRNA expression of collagen types I, III, and V; matrix metalloproteinases (MMPs) 2, 3, 9, 13, 14; and decreased mRNA expression of collagen type II in Co8-9 disc. Compression loading altered the expression of integrin alpha 2 beta 1 (upregulated) and alpha 10 beta 1 (downregulated) in NP cells, and activated integrin downstream signaling. By contrast, one-level model showed more severe disc degeneration and ECM remodeling. Integrin alpha 1, alpha 2, alpha 11, and beta 1 were upregulated, whereas alpha 10 was downregulated. Similar activation of integrin signaling was observed. Conclusion. Static compression altered collagen and MMP expression, and promoted beta 1 integrin expression and signaling in IVD. Compared with one-level rat tail IVDD model, three-level model showed milder effects on disc degeneration, ECM remodeling, and integrin expression, suggesting one-level model might involve other causes that induce IVDD via mechanisms independent of compression force.
基金：
National Basic Research Program of China (2014CB541905), National
Natural Science Foundation of China (31525016, 31190061), State Key
Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell
Biology (LMCB, KF2011002) funds were received in support of this work.
第一作者机构：[1]The Second Affiliated Hospital of Soochow University, Suzhou, China
通讯作者：
通讯机构：[*1]Institute of Biochemistry and Cell Biology, 320 YueYang Road, Shanghai 200031, China
推荐引用方式(GB/T 7714)：
ZhanJun Yan ,YouDong Pan ,ShiHui Wang ,et al.Static Compression Induces ECM Remodeling and Integrin alpha 2 beta 1 Expression and Signaling in a Rat Tail Caudal Intervertebral Disc Degeneration Model[J].SPINE.2017,42(8):E448-E458.doi:10.1097/BRS.0000000000001856.
APA：
ZhanJun Yan,,YouDong Pan,,ShiHui Wang,,MaoHua Cheng,HongMei Kong,...&JianFeng Chen,.(2017).Static Compression Induces ECM Remodeling and Integrin alpha 2 beta 1 Expression and Signaling in a Rat Tail Caudal Intervertebral Disc Degeneration Model.SPINE,42,(8)
MLA：
ZhanJun Yan,,et al."Static Compression Induces ECM Remodeling and Integrin alpha 2 beta 1 Expression and Signaling in a Rat Tail Caudal Intervertebral Disc Degeneration Model".SPINE 42..8(2017):E448-E458
