Decreased microRNA miR-181c expression in peripheral blood mononuclear cells correlates with elevated serum levels of IL-7 and IL-17 in patients with myasthenia gravis
机构：[1]Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China[2]Department of Emergency Medicine, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, Jiangsu, China[3]Department of Neurology, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu, China[4]Department of Pathogenic Biology and Lab of Infection and Immunology, Xuzhou Medical College, Xuzhou, Jiangsu, China[5]Department of Neurology, Affiliated Hospital of Jining Medical University, Jining, Shandong, China
miR-181c is a newly identified negative regulator of immune cell activation. In this study, we aimed to investigate the expression and functional role of miR-181c in myasthenia gravis (MG). miR-181c showed significant downregulation in peripheral blood mononuclear cells (PBMCs) from MG patients compared with healthy controls, with lower expression in generalized patients than in ocular ones. MG patients also had increased serum IL-7 and IL-17 levels. Additionally, serum IL-7 level presents a positive correlation with the serum IL-17 level. miR-181c levels were negatively correlated with serum levels of IL-7 and IL-17 in either generalized patients or ocular patients. A luciferase reporter assay revealed that miR-181c could directly bind to the 3'-UTR of interleukin-7. Forced expression of miR-181c led to decreased IL-7 and IL-17 release in cultured PBMCs, while depletion of miR-181c increased the secretion of these two proinflammatory cytokines. The results from our study suggested for the first time that miR-181c was able to negatively regulate the production of proinflammatory cytokines IL-7 and IL-17 in MG patients, and it is a novel potential therapeutic target for MG.
基金：
This work was supported by
National Nature Science Foundation of China (81072465), Natural
Science Fund of the Educational Committee of Jiangsu Province
(10KJD320003), Special foundation of president of the Xuzhou
Medical College (2010KJZ01), Key medical talents fund of Jiangsu
Province (H201130), Jiangsu Province ordinary university postgraduate
research innovation fund (CXLX11_0734).
第一作者机构：[1]Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China
推荐引用方式(GB/T 7714)：
Yong Zhang,Mingfeng Guo,Ning Xin,et al.Decreased microRNA miR-181c expression in peripheral blood mononuclear cells correlates with elevated serum levels of IL-7 and IL-17 in patients with myasthenia gravis[J].CLINICAL AND EXPERIMENTAL MEDICINE.2016,16(3):413-21.doi:10.1007/s10238-015-0358-1.
APA：
Yong Zhang,Mingfeng Guo,Ning Xin,Zhen Shao,Xiuying Zhang...&Deqin Geng.(2016).Decreased microRNA miR-181c expression in peripheral blood mononuclear cells correlates with elevated serum levels of IL-7 and IL-17 in patients with myasthenia gravis.CLINICAL AND EXPERIMENTAL MEDICINE,16,(3)
MLA：
Yong Zhang,et al."Decreased microRNA miR-181c expression in peripheral blood mononuclear cells correlates with elevated serum levels of IL-7 and IL-17 in patients with myasthenia gravis".CLINICAL AND EXPERIMENTAL MEDICINE 16..3(2016):413-21
