机构：[1]Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University, Suzhou,China,[2]Institute of Radiotherapy & Oncology, Soochow University, Suzhou, China,[3]Suzhou Key Laboratory for Radiation Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China
Objective: To characterize the early delayed and late-delayed cognitive dysfunction induced by various doses of whole brain irradiation in young rats.Methods: One-month-old Sprague-Dawley male rats were divided randomly into the 0 (control), 0 (anesthesia control), 2, 10, 20, and 30-Gy groups. Each group was then subdivided into 4 groups according to the experimental intervals: 1, 2, 3, and 6months after radiation. Rats were irradiated using a 4-MeV electron beam, which was generated by a linear accelerator. Sequential behavioral tests, including open field, novel location and novel object recognition and Morris water maze were performed after radiation. Changes in gross neurological symptoms, body weight, topical skin response, and histopathology were observed.Results: In the open field test, there were no radiation-induced alterations found. In the novel location and novel object recognition tests, rats of the 20-Gy group spent less time exploring the novel object and novel location 3months after irradiation. During the place navigation test, the spatial working memory of the 30 and 20-Gy irradiated rats were impaired from 1 to 2months after irradiation, respectively. In the spatial probe test, the 20 and 30-Gy irradiated rats spent less time in the critical region compared to control rats at 3 and 6months post-irradiation. Morphological changes, including edema, vascular dilation, focal necrosis, demyelination, and adjacent reactive gliosis were observed in the 30-Gy irradiation group.Conclusion: More than 20Gy of whole brain irradiation dose can cause significant cognitive dysfunction in young rats.
基金：
This work was supported by the National Natural
Science Foundation of China [grant number 81472804],
[grant number 81172128]; Graduate Innovation Project
of Jiangsu Province [grant number CXZZ13_0838];
Jiangsu Provincial Special Program of Clinical Medical
Science [grant number BL2014040]; Suzhou Science
and Technology Development Program [grant number
SZS201509]; National Natural Scientific Foundation of
China [grant number 81372411].
第一作者机构：[1]Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University, Suzhou,China,
通讯作者：
通讯机构：[*1]Suzhou Key Laboratory for Radiation Oncology, The Second Affiliated Hospital of Soochow University, Suzhou,215004, China.
推荐引用方式(GB/T 7714)：
Rui Sun,Li-Yuan Zhang,Lie-Song Chen,et al.Long-term outcome of changes in cognitive function of young rats after various/different doses of whole brain irradiation[J].NEUROLOGICAL RESEARCH.2016,38(7):647-54.doi:10.1080/01616412.2016.1188483.
APA：
Rui Sun,Li-Yuan Zhang,Lie-Song Chen&Ye Tian.(2016).Long-term outcome of changes in cognitive function of young rats after various/different doses of whole brain irradiation.NEUROLOGICAL RESEARCH,38,(7)
MLA：
Rui Sun,et al."Long-term outcome of changes in cognitive function of young rats after various/different doses of whole brain irradiation".NEUROLOGICAL RESEARCH 38..7(2016):647-54
