机构：[a]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China[b]State Key Laboratory of Reproductive Medicine, Department of Prenatal Diagnosis, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China[c]Cam-Su Genomic Resource Center, Soochow University, Suzhou 215123, China[d]Orthopedic Department, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China
Cardiac cell apoptosis provoked by excessive sodium nitroprusside (SNP) toxicity, a potent vasodilator, limited its clinical application. Effective means for protection against SNP-induced cardiotoxicity would be highly needed. This study investigated the effects of Follistatin-like 1 (FSTL1) on the injury induced by SNP in rat cardiomyoblast H9c2 cells. First, expression of FSTL is attenuated following SNP treatment. SNP challenge significantly increases cardiac cell death, which is attenuated by FSTL1 pretreatment. Additionally, knockdown of endogenous FSTL1 enhances SNP-induced cell apoptosis. Furthermore, FSTL1 pretreatment partially inhibits SNP-induced NO generation. LY294002 and BMP4 completely abolish cytoprotective role of FSTL1 against SNP challenge, indicating both activation of Akt and inhibition of BMP/Smad1/5/9 signaling are involved in this cellular process. Lastly, FSTL1-mediated cytoprotection is independent of Smad2/3 signaling, as SB525334 fails to remove its protective role. Taken together, these results indicated that FSTL1 protects the SNP-induced injury in cardiac-H9c2 cells through, at least in part, the activation of Akt and inhibition of Smad1/5/9 signaling. (C) 2015 Elsevier Inc. All rights reserved.
基金：
This work was supported by National Natural Science Foundation
of China (NSFC-31401239 & NSFC-81300495), Jiangsu Province's
Natural Science Foundation for Colleges and Universities
(14KJB180021), Jiangsu Province's Key Discipline/Laboratory of
Medicine (XK201118), and Project on Social Development of Jiangsu
Province (BE2015614).
第一作者机构：[a]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China
共同第一作者：
通讯作者：
通讯机构：[a]Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China
推荐引用方式(GB/T 7714)：
Weiqian Chen,Jun Xia,Ping Hu,et al.Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2016,469(3):418-23.doi:10.1016/j.bbrc.2015.12.026.
APA：
Weiqian Chen,Jun Xia,Ping Hu,Fei Zhou,Yueqiu Chen...&Zhenya Shen.(2016).Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,469,(3)
MLA：
Weiqian Chen,et al."Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 469..3(2016):418-23
