机构：[1]Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China
Recent studies suggest that SATB1 is a promising therapeutic target for prostate cancer. To develop novel SATB1-based therapeutic agents for prostate cancer, in this study, we aimed to construct ZD55-SATB1, an oncolytic adenovirus ZD55 carrying shRNA targeting SATB1, and investigate its effects on the inhibition of prostate cancer growth and metastasis. ZD55-SATB1 was constructed and used to infect human prostate cancer cell lines DU145 and LNCaP. The inhibitory effect of ZD55-SATB1 on SATB1 expression was evaluated by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. The cytotoxicity of ZD55-SATB1 was detected by MTT assay. Cell invasion was detected by Matrigel invasion assay. The in vivo antitumor activities of ZD55-SATB1 were evaluated in xenograft mouse model. We found that ZD55-SATB1 selectively replicated and significantly reduced SATB1 expression in DU145 and LNCaP cells. ZD55-SATB1 effectively inhibited the viability and invasion of DU145 and LNCaP cells in vitro and inhibited prostate cancer growth and metastasis in xenograft nude mice. In conclusion, replicative oncolytic adenovirus armed with SATB1 shRNA exhibits effective antitumor effect in human prostate cancer. Our study provides the basis for the development of ZD55-SATB1 for the treatment of prostate cancer.
基金：
This study was supported by grant from the National
Natural Science Foundation of China (No. 3070099).
第一作者机构：[1]Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China
推荐引用方式(GB/T 7714)：
Li-jun Mao,Jie Zhang,Ning Liu,et al.Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis[J].TUMOR BIOLOGY.2015,36(11):9073-81.doi:10.1007/s13277-015-3658-x.
APA：
Li-jun Mao,Jie Zhang,Ning Liu,Li Fan,Dong-rong Yang...&Jun-nian ZHENG.(2015).Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis.TUMOR BIOLOGY,36,(11)
MLA：
Li-jun Mao,et al."Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis".TUMOR BIOLOGY 36..11(2015):9073-81
