Objective: The aim of this study was to explore the role of autophagy on the regulation of endothelial progenitor cells (EPCs) migration under normoxic condition. Methods: After EPCs were isolated and characterized in vitro, we employed Atg5 knocking down and rapamycin to monitor the autophagy, and performed wound healing and transwell assay to assess the cell migration. On the mechanism, the expression of matrix metalloproteinases (MMPs) and urokinase type plasminogen activator (uPA) was evaluated. Results: Atg5 knocking down and rapamycin could respectively inhibit and enhance autophagy, which could result in significantly increased and decreased cell migration in wound healing and transwell assay under normoxic condition. Moreover, Atg5 knocking down could significantly increase the expression of MMP2, MMP9 and uPA in EPCs while rapamycin could decrease the expression of uPA and MMP9. In addition, the mTOR-P70 S6K pathway was also involved in EPCs migration regulation. Conclusions: These results demonstrated that autophagy could regulate the EPCs migration through mTOR-P70 S6K pathway, and MMP2, MMP9 and uPA may also involve in the regulation mechanism. (C) 2015 Elsevier Inc. All rights reserved.
基金：
This work was supported by grants from the National Natural
Science Foundation of China (No. 81400345, No. 30972941), Jiangsu
Provincial Health Department's Medical Science Program
(H201211).Graduate Research and Innovation Program in Colleges
and Universities of Jiangsu Province (No. 1202).
第一作者机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, 1055, Sanxiang Rd, Suzhou 215000, Jiangsu, China.
推荐引用方式(GB/T 7714)：
Wen-Dong Li,Nan Hu,Feng-Rui Lei,et al.Autophagy inhibits endothelial progenitor cells migration via the regulation of MMP2, MMP9 and uPA under normoxia condition[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,466(3):376-80.doi:10.1016/j.bbrc.2015.09.031.
APA：
Wen-Dong Li,Nan Hu,Feng-Rui Lei,Sen Wei,Jian-Jie Rong...&Xiao-Qiang Li.(2015).Autophagy inhibits endothelial progenitor cells migration via the regulation of MMP2, MMP9 and uPA under normoxia condition.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,466,(3)
MLA：
Wen-Dong Li,et al."Autophagy inhibits endothelial progenitor cells migration via the regulation of MMP2, MMP9 and uPA under normoxia condition".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 466..3(2015):376-80
