机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou, 215004, People’s Republic of China[2]Department of Medical Equipment, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou, 215004, People’s Republic of China
Immunological T cells and associated cytokines have been shown to be involved in the pathogenesis of multiple myeloma (MM). However, the abnormal immune imbalance of T lymphocyte subsets on MM remains unknown. We investigate the proportions of T helper 1 (Th1)/Th2/Th17/T regulatory (Treg) cells in peripheral blood mononuclear cells (PBMCs) by flow cytometry (FCM), and serum levels of relevant cytokines in MM patients and controls were detected by enzyme-linked immunosorbent assay (ELISA). The messenger RNA (mRNA) expression of T-bet, STAT6, RORgammat, and Foxp3 was measured by real-time quantitative polymerase chain reaction (PCR). The CD4+ Th1 and CD4+ Th17 cells in patients with MM were significantly higher than those in health controls as well as the expression of T-bet and RORgammat mRNA. Furthermore, serum levels of interferon gamma (IFN-gamma), IL-6, and IL-17A in MM group were greatly increased and significantly associated with each other. Significant differences on Th cells, cytokines, and transcription factors were observed on MM patients. The imbalance of T lymphocyte subsets was thought to contribute to the pathogenesis and underlying mechanisms of MM.
基金：
This research was supported by a grant from the National
Natural Science Foundation of China (81301494).
第一作者机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou, 215004, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Medical Equipment, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou, 215004, People’s Republic of China
推荐引用方式(GB/T 7714)：
Ping Feng,Ruhong Yan,Xiaoli Dai,et al.The Alteration and Clinical Significance of Th1/Th2/Th17/Treg Cells in Patients with Multiple Myeloma[J].INFLAMMATION.2015,38(2):705-9.doi:10.1007/s10753-014-9980-4.
APA：
Ping Feng,Ruhong Yan,Xiaoli Dai,Xiaofang Xie,Huiyan Wen&Shun Yang.(2015).The Alteration and Clinical Significance of Th1/Th2/Th17/Treg Cells in Patients with Multiple Myeloma.INFLAMMATION,38,(2)
MLA：
Ping Feng,et al."The Alteration and Clinical Significance of Th1/Th2/Th17/Treg Cells in Patients with Multiple Myeloma".INFLAMMATION 38..2(2015):705-9
