机构：[a]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, China[b]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, China[c]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, College of Pharmaceutical Sciences, Soochow University, Suzhou, China[d]Department of Anesthesiology & Peri-operative Medicine, Oregon Health & Science University, Portland, OR, USA
How hydrogen sulfide (H2S) protects against myocardial ischemia-reperfusion (I/R) injury is poorly understood. By using a slow-releasing H2S donor, we investigated if H2S protected against myocardial I/R injury by activating AMPK and restoring I/R-impaired autophagic flux. Male rats received anterior descending coronary artery occlusion followed by reperfusion. The H2S donor ADT and/or the AMPK inhibitor, compound C (CC), were administered after occlusion. Infarction was analyzed histologically. AMPK activation was assessed in the ischemic heart by analyzing phosphorylation of AMPK and S6 ribosomal protein. Autophagy was assessed by analyzing the following markers: microtubule-associated protein 1 light chain 3 (LC3) I and II, lysosome associated membrane protein-2 (LAMP-2), P62 and beclin-1. We further investigated if blocking autophagic flux with chloroquine abolished ADT cardioprotection in vivo. Myocardial I/R reduced serum H2S levels, which was elevated by ADT. ADT enhanced AMPK activation and reduced infarction following I/R, and both effects were abolished by AMPK inhibition. Myocardial I/R induced autophagosome accumulation, as evidenced by the increased ratios of LC3-II/LC3-I, upregulation of beclin-1 and P62 and reduction in LAMP-2. ADT blunted these autophagic changes induced by I/R, indicating that ADT restored I/R-impaired autophagic flux. The AMPK inhibitor CC blocked ADT effects on restoring I/R-impaired autophagy flux. Moreover, chloroquine pretreatment abolished cardioprotection of ADT and increased autophagosome accumulation in the ADT-treated heart following I/R. In conclusion, AMPK activation and subsequent restoration of I/R-impaired autophagic flux are unrecognized mechanisms underlying cardioprotective effects conferred by H2S donors. (C) 2015 Elsevier Inc. All rights reserved.
基金：
The project is supported by the grants from National Science
Foundation of China (81471336, 81371278, 81171246), the Natural
Science Foundation of Jiangsu Province of China (Grant No:
BK20141187), the Priority Academic Program Development of
Jiangsu Higher Education Institutions of China, Science and Technology
Program from Suzhou City (Grant No: SYS201317).
第一作者机构：[a]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, China[b]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, China
共同第一作者：
通讯作者：
通讯机构：[b]Department of Anesthesiology, The Second Affiliated Hospital of Soochow University, China[*1]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, Soochow University, 199 Renai Road, Suzhou, Jiangsu Province 215123, China.
推荐引用方式(GB/T 7714)：
Hong Xie,Qingrong Xu,Jia Jia,et al.Hydrogen sulfide protects against myocardial ischemia and reperfusion injury by activating AMP-activated protein kinase to restore autophagic flux[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,458(3):632-638.doi:10.1016/j.bbrc.2015.02.017.
APA：
Hong Xie,Qingrong Xu,Jia Jia,Guizhen Ao,Ying Sun...&Jian Cheng.(2015).Hydrogen sulfide protects against myocardial ischemia and reperfusion injury by activating AMP-activated protein kinase to restore autophagic flux.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,458,(3)
MLA：
Hong Xie,et al."Hydrogen sulfide protects against myocardial ischemia and reperfusion injury by activating AMP-activated protein kinase to restore autophagic flux".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 458..3(2015):632-638
