机构：[a]Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China[b]Institute of Neuroscience, Soochow University, Suzhou 215123, China[c]Department of Pharmacology, School of Pharmaceutical Science, Soochow University, Suzhou 215123, China[d]Center for Circadian Clock, Soochow University, Suzhou, China[e]School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China
It has been demonstrated that acid sensing ionic channels (ASICs) are present in the central and peripheral nervous system of mammals, including the retina. However, it remains unclear whether the zebrafish retina also expresses ASICs. In the present study, the expression and distribution of zasic1 were examined in the retina of zebrafish. Both zasic1 mRNA and protein expressions were detected in the adult zebrafish retina. A wide distribution of ASIC1 in zebrafish retina was confirmed using whole mount in situ hybridization and immunohistochemistry study. Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were also recorded using whole cell patch clamping. Moreover, blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure. In sum, our data demonstrate the presence of ASIC1 and its possible functional relevance in the retina of zebrafish. (C) 2014 Elsevier Inc. All rights reserved.
基金：
This work was supported by the National Natural Science
Foundation of China (81471299, 81301090), the Research Fund
for the Doctoral Program of Higher Education of China (RFDP,
20123201110015), Suzhou Science and Technology Development
Program (SYS201126), the Priority Academic Program Development
of Jiangsu Higher Education Institutions (PAPD), and the
Plans for Graduate Research and Innovation in Colleges and Universities
of Jiangsu Province (CXZZ13_0833).
第一作者机构：[a]Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China[b]Institute of Neuroscience, Soochow University, Suzhou 215123, China
共同第一作者：
通讯作者：
通讯机构：[b]Institute of Neuroscience, Soochow University, Suzhou 215123, China
推荐引用方式(GB/T 7714)：
Sha Liu,Mei-Xia Wang,Cheng-Jie Mao,et al.Expression and functions of ASIC1 in the zebrafish retina[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2014,455(3-4):353-7.doi:10.1016/j.bbrc.2014.11.021.
APA：
Sha Liu,Mei-Xia Wang,Cheng-Jie Mao,Xiao-Yu Cheng,Chen-Tao Wang...&Chun-Feng Liu.(2014).Expression and functions of ASIC1 in the zebrafish retina.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,455,(3-4)
MLA：
Sha Liu,et al."Expression and functions of ASIC1 in the zebrafish retina".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 455..3-4(2014):353-7
