机构：[a]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China[b]Department of Orthopedics, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi 214000, China[c]Institute of Neuroscience, University of Oregon, Eugene, OR, USA[d]Osteoporosis Diagnosis and Treatment Technology, Institute of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China
Iron overload, as a risk factor for osteoporosis, can result in the up-regulation of Hepcidin, and Hepcidin knockout mice display defects in their bone microarchitecture. However, the molecular and genetic mechanisms underlying Hepcidin deficiency-derived bone loss remain unclear. Here, we show that hepcidin knockdown in zebrafish using morpholinos leads to iron overload. Furthermore, a mineralization delay is observed in osteoblast cells in hepcidin morphants, and these defects could be partially restored with microinjection of hepcidin mRNA. Quantitative real-time PCR analyses revealed the osteoblast-specific genes alp, runx2a, runx2b, and sp7 in morphants are down-regulated. Furthermore, we confirmed qRT-PCR results by in situ hybridization and found down-regulated genes related to osteoblast function in hepcidin morphants. Most importantly, we revealed that hepcidin was capable of removing whole-body iron which facilitated larval recovery from the reductions in bone formation and osteogenesis induced by iron overload. (C) 2016 Elsevier Inc. All rights reserved.
基金：
This work was supported in part by grants from the National
Natural Science Foundation of China (81273090,81302438,
8160120405), Jiangsu Province Special Medical Grant (BL2014044),
Suzhou Clinical Key Special Diseases Fund (LCZX210305), Research
and Innovation Project for College Graduates of Jiangsu Province
(KYLX_1264).
第一作者机构：[a]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China[b]Department of Orthopedics, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi 214000, China[d]Osteoporosis Diagnosis and Treatment Technology, Institute of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China
通讯作者：
通讯机构：[a]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China[b]Department of Orthopedics, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi 214000, China
推荐引用方式(GB/T 7714)：
Yu Jiang,Yilin Yan,Xiao Wang,et al.Hepcidin inhibition on the effect of osteogenesis in zebrafish[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2016,476(1):1-6.doi:10.1016/j.bbrc.2016.05.118.
APA：
Yu Jiang,Yilin Yan,Xiao Wang,Guoxing Zhu&You-Jia Xu.(2016).Hepcidin inhibition on the effect of osteogenesis in zebrafish.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,476,(1)
MLA：
Yu Jiang,et al."Hepcidin inhibition on the effect of osteogenesis in zebrafish".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 476..1(2016):1-6
