机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China[2]Institute of Neuroscience, University of Oregon, Eugene, OR, USA[3]Center for Circadian Clock, Soochow University, Suzhou, China
Osteoporosis results from an imbalance in bone remodeling, in which osteoclastic bone resorption exceeds osteoblastic bone formation. Iron has recently been recognized as an independent risk factor for osteoporosis. Reportedly, excess iron could promote osteoclast differentiation and bone resorption through the production of reactive oxygen species (ROS). We evaluated the effect of iron on osteoblast differentiation and bone formation in zebrafish and further investigated the potential benefits of deferoxamine (DFO), a powerful iron chelator, in iron-overloaded zebrafish. The zebrafish model of iron overload described in this study demonstrated an apparent inhibition of bone formation, accompanied by decreased expression of osteoblast-specific genes (runx2a, runx2b, osteocalcin, osteopontin, ALP, and collagen type I). The negative effect of iron on osteoblastic activity and bone formation could be attributed to increased ROS generation and oxidative stress. Most importantly, we revealed that DFO was capable of removing whole-body iron and attenuating oxidative stress in iron-overloaded larval zebrafish, which facilitated larval recovery from the reductions in bone formation and osteogenesis induced by iron overload.
基金：
This work was supported in part by the National
Natural Science Foundation of China (81273090), a Jiangsu Provincial
Grant (BK2012608), Science and Technology Projects of Suzhou
(SS201327), and the Foundation Program of Suzhou Key Laboratory
of High Technology (SZS201208).
第一作者机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China
通讯作者：
通讯机构：[1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China
推荐引用方式(GB/T 7714)：
Bin Chen,Yi-Lin Yan,Chen Liu,et al.Therapeutic Effect of Deferoxamine on Iron Overload-Induced Inhibition of Osteogenesis in a Zebrafish Model[J].CALCIFIED TISSUE INTERNATIONAL.2014,94(3):353-60.doi:10.1007/s00223-013-9817-4.
APA：
Bin Chen,Yi-Lin Yan,Chen Liu,Lin Bo,Guang-Fei Li...&You-Jia Xu.(2014).Therapeutic Effect of Deferoxamine on Iron Overload-Induced Inhibition of Osteogenesis in a Zebrafish Model.CALCIFIED TISSUE INTERNATIONAL,94,(3)
MLA：
Bin Chen,et al."Therapeutic Effect of Deferoxamine on Iron Overload-Induced Inhibition of Osteogenesis in a Zebrafish Model".CALCIFIED TISSUE INTERNATIONAL 94..3(2014):353-60
