机构：[a]Department of Orthopedics, The Affiliated Jiangyin Hospital of Medical College of Southeast University, Jiangyin City, Jiangsu 214400, China[b]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China
The search for novel and more efficient chemo-agents against malignant osteoblastoma is important. In this study, we examined the potential anti-osteoblastoma function of bufotalin, and studied the underlying mechanisms. Our results showed that bufotalin induced osteoblastoma cell death and apoptosis in dose- and time-dependent manners. Further, bufotalin induced endoplasmic reticulum (ER) stress activation in osteoblastoma cells, the latter was detected by the induction of C/EBP homologous protein (CHOP), phosphorylation of inositol-requiring enzyme 1 (IRE1) and PKR-like endoplasmic reticulum kinase (PERK), as well as caspase-12 activation. Conversely, the ER stress inhibitor salubrinal, the caspase-12 inhibitor z-ATAD-fmk as well as CHOP depletion by shRNA significantly inhibited bufotalin-induced osteoblastoma cell death and apoptosis. Finally, by using a mice xenograft model, we demonstrated that bufotalin inhibited U2OS osteoblastoma cell growth in vivo. In summary, our results suggest that ER stress contributes to bufotalin-induced apoptosis in osteoblastoma cells. Bufotalin might be investigated as a novel anti-osteoblastoma agent. (C) 2014 Elsevier Inc. All rights reserved.
第一作者机构：[a]Department of Orthopedics, The Affiliated Jiangyin Hospital of Medical College of Southeast University, Jiangyin City, Jiangsu 214400, China
共同第一作者：
通讯作者：
通讯机构：[*]Department of Orthopedics, The Affiliated Jiangyin Hospital of Medical College of Southeast University, No. 163 Shoushan Rd, Jiangyin City, Jiangsu 215600, China
推荐引用方式(GB/T 7714)：
Yun-Rong Zhu,Yong Xu,Jian-Feng Fang,et al.Bufotalin-induced apoptosis in osteoblastoma cells is associated with endoplasmic reticulum stress activation[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2014,451(1):112-8.doi:10.1016/j.bbrc.2014.07.077.
APA：
Yun-Rong Zhu,Yong Xu,Jian-Feng Fang,Feng Zhou,Xiong-Wei Deng&Yun-Qing Zhang.(2014).Bufotalin-induced apoptosis in osteoblastoma cells is associated with endoplasmic reticulum stress activation.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,451,(1)
MLA：
Yun-Rong Zhu,et al."Bufotalin-induced apoptosis in osteoblastoma cells is associated with endoplasmic reticulum stress activation".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 451..1(2014):112-8
