N-Succinyl-chitosan nanoparticles coupled with low-density lipoprotein for targeted osthole-loaded delivery to low-density lipoprotein receptor-rich tumors
机构：[1]Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou,[2]The Children’s Hospital of Wuxi People’s Hospital, Nanjing Medical University, Wuxi,[3]College of Radiological Medicine and Protection, Soochow University, Suzhou,[4]Changshu Hospital of Traditional Chinese Medicine, Changshu,[5]Invasive Technology Department, The Second Affiliated Hospital of Soochow University, Suzhou
N-Succinyl-chitosan (NSC) was synthesized and NSC nanoparticles (NPs) with loaded osthole (Ost) (Ost/NSC-NPs) were prepared by emulsion solvent diffusion. Subsequently, low-density lipoprotein (LDL)-mediated NSC-NPs with loaded Ost (Ost/LDL-NSC-NPs) were obtained by coupling LDL with Ost/NSC-NPs through amide linkage. The average particle size of Ost/NSC-NPs was approximately 145 nm, the entrapment efficiency was 78.28%+/- 2.06%, and the drug-loading amount was 18.09%+/- 0.17%. The release of Ost from Ost/NSC-NPs in vitro showed a more evident sustained effect than the native material. The half maximal inhibitory concentration of Ost/LDL-NSC-NPs was only 16.23% that of the free Ost at 24 hours in HepG2 cells. Ost inhibited HepG2 cell proliferation by arresting cells in the synthesis phase of the cell cycle and by triggering apoptosis. Cellular uptake and subcellular localization in vitro and near-infrared fluorescence real-time imaging in vivo showed that Ost/LDL-NSC-NPs had high targeting efficacy. Therefore, LDL-NSC-NPs are a promising system for targeted Ost delivery to liver tumor.
基金：
This work was supported by the National Natural Science Foundation of China projects (No 81273463), the Jiangsu Science and Technology Support Plan (BE2011670), the National Student and Innovative experimental plan (111028533, microcomputer number: 5731503311), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
第一作者机构：[1]Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou,
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Pharmaceutics, College of Pharmaceutical Science, Soochow University, DuShuHu High Education Zone, Suzhou, Jiang Su Province 215123[*2]Invasive Technology Department, The Second Affiliated Hospital of Soochow University, SanXiang Road 1055, Suzhou, Jiang Su Province 215004
推荐引用方式(GB/T 7714)：
Chun-ge Zhang,Qiao-ling Zhu,Yi Zhou,et al.N-Succinyl-chitosan nanoparticles coupled with low-density lipoprotein for targeted osthole-loaded delivery to low-density lipoprotein receptor-rich tumors[J].INTERNATIONAL JOURNAL OF NANOMEDICINE.2014,9(1):2919-32.doi:10.2147/IJN.S59799.
APA：
Chun-ge Zhang,Qiao-ling Zhu,Yi Zhou,Yang Liu,Wei-liang Chen...&Yong Jin.(2014).N-Succinyl-chitosan nanoparticles coupled with low-density lipoprotein for targeted osthole-loaded delivery to low-density lipoprotein receptor-rich tumors.INTERNATIONAL JOURNAL OF NANOMEDICINE,9,(1)
MLA：
Chun-ge Zhang,et al."N-Succinyl-chitosan nanoparticles coupled with low-density lipoprotein for targeted osthole-loaded delivery to low-density lipoprotein receptor-rich tumors".INTERNATIONAL JOURNAL OF NANOMEDICINE 9..1(2014):2919-32
医学