机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China[3]Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA
Polydatin (PD), a small natural compound from Polygonum cuspidatum, has a number of biological functions. However, the anticancer activity of PD has been poorly investigated. In the present study, thiazolyl blue tetrazolium bromide assay was used to evaluate the inhibitory effect of PD on cell growth. Cell cycle distribution and apoptosis were investigated by flow cytometry. In addition, the expression of several proteins associated with apoptosis and cell cycle were analyzed by western blot analysis. The results demonstrated that PD significantly inhibits the proliferation of A549 and NCI-H1975 lung cancer cell lines and causes dose-dependent apoptosis. Cell cycle analysis revealed that PD induces S phase cell cycle arrest. Western blot analysis showed that the expression of Bcl-2 decreased as that of Bax increased, and the expression of cyclin D1 was also suppressed. The results suggest that PD has potential therapeutic applications in the treatment of lung cancer.
基金：
The current study was supported by grants from the National Natural Science Foundation of China (nos. 81071906 and 81172127), Suzhou Key Laboratory of Radiation Oncology (no. SZS0802), Suzhou Science and Technology Program (SYS201345) and the Priority Academic Program Development (PAPD) of Jiangsu Higher education institutions
第一作者机构：[1]Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China
共同第一作者：
通讯作者：
通讯机构：[*1]Laboratory of Tumor Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, 199 Renai Road, Suzhou, Jiangsu 215123, P.R. China
推荐引用方式(GB/T 7714)：
YUSONG ZHANG,ZHIXIANG ZHUANG,QINGHUI MENG,et al.Polydatin inhibits growth of lung cancer cells by inducing apoptosis and causing cell cycle arrest[J].ONCOLOGY LETTERS.2014,7(1):295-301.doi:10.3892/ol.2013.1696.
APA：
YUSONG ZHANG,ZHIXIANG ZHUANG,QINGHUI MENG,YANG JIAO,JIAYING XU&SAIJUN FAN.(2014).Polydatin inhibits growth of lung cancer cells by inducing apoptosis and causing cell cycle arrest.ONCOLOGY LETTERS,7,(1)
MLA：
YUSONG ZHANG,et al."Polydatin inhibits growth of lung cancer cells by inducing apoptosis and causing cell cycle arrest".ONCOLOGY LETTERS 7..1(2014):295-301
