机构：[1]Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou 215123, China[3]Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou 215123, China
Bladder urothelial carcinoma (BUC) accounts for approximate to 90% of all cases of bladder cancer. Reduced expression of TGFBR3 has been frequently observed in several types of human cancers. However, little is known about whether expression of TGFBR3 reduced in BUC and the underlying mechanisms. In the present study, we performed quantitative real-time PCR to examine the mRNA expression of TGFBR3 and GATA3, and bisulfite genomic sequencing to evaluate the methylation status in TGFBR3 and GATA3 promoter regions in fresh tumor and the corresponding paracarcinoma tissues from 29 patients with BUC. As a result, the expression of TGFBR3 and GATA3, a transcriptional factor of the TGFBR3 gene, were found to be co-downregulated in BUC. Moreover, our findings indicated that GATA3 promoter methylation was one of the reasons for silencing of GATA3 and TGFBR3 in BUC, albeit TGFBR3 methylation and mutation were not associated with reduced expression of TGFBR3 mRNA in BUC. In summary, our findings suggest that methylation in the GATA3 promoter region may inhibit the expression of GATA3 mRNA, which leads to the reduced expression of TGFBR3 mRNA in BUC. Anat Rec, 296:1717-1723, 2013. (c) 2013 Wiley Periodicals, Inc.
基金：
Grant sponsor: National Natural Science Foundation of
China; Grant number: 81171894; Grant sponsor: Program for
New Century Excellent Talents in University; Grant number:
NCET-09-0165; Grant sponsor: Suzhou Key Laboratory for
Molecular Cancer Genetics; Grant number: SZS201209.; Grant
sponsor: Suzhou Science and Education Youth Health Foundation;
Grant number: KJXW2012014; Grant sponsor: Scientific
Innovation Research of College Graduate of Jiangsu Province;
Grant number: CXZZ12_0844; Grant sponsor: “333” Project of
Jiangsu Province Government, Soochow Scholar Project of Soochow
University.
第一作者机构：[1]Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Urology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.[*2]Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, 199 Ren’ai Road, Sino-Singapore Industrial Park, Suzhou 215123, China.
推荐引用方式(GB/T 7714)：
XIAO-LONG LIU ,BO-XIN XUE ,ZHE LEI ,et al.TGFBR3 Co-Downregulated With GATA3 Is Associated With Methylation of the GATA3 Gene in Bladder Urothelial Carcinoma[J].ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY.2013,296(11):1717-23.doi:10.1002/ar.22802.
APA：
XIAO-LONG LIU,,BO-XIN XUE,,ZHE LEI,,DONG-RONG YANG,,QING-CHUAN ZHANG,...&HONG-TAO ZHANG.(2013).TGFBR3 Co-Downregulated With GATA3 Is Associated With Methylation of the GATA3 Gene in Bladder Urothelial Carcinoma.ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY,296,(11)
MLA：
XIAO-LONG LIU,,et al."TGFBR3 Co-Downregulated With GATA3 Is Associated With Methylation of the GATA3 Gene in Bladder Urothelial Carcinoma".ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY 296..11(2013):1717-23
