机构：[1]Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, Jiangsu 215123[3]Suzhou Key Laboratory for Molecular Cancer Genetics, Suzhou, Jiangsu 215123, P.R. China
Bladder cancer is one of the most common genitourinary malignant diseases worldwide. More than 90% of bladder cancer cases are bladder urothelial carcinoma (BUC). Although transforming growth factor-beta III receptor (TGFBR3) has been suggested to play a dual role in cancer progression, little is known about TGFBR3 in bladder cancer. In the present study, fresh tumor and the corresponding paracarcinoma tissue specimens were collected from 56 bladder urothelial carcinoma patients. TGFBR3 expression in these tissues was determined by western blotting. TGFBR3 was also detected in the human normal urothelial cell line SV-HUC-1, the human superficial urothelial bladder cancer cell line 5637, and the human invasive bladder cancer cell line T24 using western blotting and quantitative PCR. Cell growth, motility and invasion were also analyzed in the control and the TGFBR3 gene-silenced T24 cells. As a result, the expression of TGFBR3 was reduced (18/30) in most superficial bladder urothelial carcinoma tissues compared to the corresponding normal tissues, whereas TGFBR3 expression was more enhanced (19/26) in the invasive samples. Similarly, an increase of TGFBR3 expression was found in T24 cells, but a decrease was observed in 5637 cells. Knockdown of TGFBR3 in T24 cells resulted in decreased cell growth, motility and invasion. In conclusion, these findings suggest that TGFBR3 may play a dichotomous role in human bladder cancer, acting as both a tumor suppressor and as a tumor promoter.
基金：
The present study was in part supported by grants from the National Natural Science Foundation of China (81171894 to H.T.Z.), the Program for New Century Excellent Talents in University (NCET-09-0165 to H.T.Z.), the ‘333’ Project of Jiangsu Province Government (to H.T.Z.), the Soochow Scholar Project of Soochow University (to H.T.Z), the Suzhou Key Laboratory for Molecular Cancer Genetics (SZS201209 to H.T.Z), the Suzhou Science and Education Youth Health Foundation (KJXW2012014 to X.L.L.) and the Scientific Innovation Research of College Graduate of Jiangsu Province (CXZZ12_0844 to X.L.L.).
第一作者机构：[1]Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Urology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu 215004, P.R. China[*2]Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, 199 Ren'ai Road, Sino-Singapore Industrial Park, Suzhou, Jiangsu 215123, P.R. China
推荐引用方式(GB/T 7714)：
XIAO-LONG LIU,KEBING XIAO,BOXIN XUE,et al.Dual role of TGFBR3 in bladder cancer[J].ONCOLOGY REPORTS.2013,30(3):1301-8.doi:10.3892/or.2013.2599.
APA：
XIAO-LONG LIU,KEBING XIAO,BOXIN XUE,DONGRONG YANG,ZHE LEI...&HONG-TAO ZHANG.(2013).Dual role of TGFBR3 in bladder cancer.ONCOLOGY REPORTS,30,(3)
MLA：
XIAO-LONG LIU,et al."Dual role of TGFBR3 in bladder cancer".ONCOLOGY REPORTS 30..3(2013):1301-8
