机构：[1]Neurosurgery Department, The Second Affiliated Hospital of Soochow University, Suzhou, China.[2]Cardiovascular Department, The Second Affiliated Hospital of Soochow University, Suzhou, China.[3]Changyi People’s Hospital, Changyi, China.[4]Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou, China.[5]South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.[6]Radiology Department, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Doxorubicin (Dox) is widely used for the treatment of solid tumors but its clinical utility on glioma is limited. In this study, we developed a novel nano-scale drug delivery system employing biodegradable nanoparticle (NP) as carriers to load Dox. Transferrin (Tf) was conjugated to the surface of NP to specifically target the NP to glioma. Tf-NP-Dox was prepared via emulsification-solvent evaporation method, and characterized for the size, Drug loading capacity (DLC), entrapment efficiency, and Tf number on the surface. The antitumor efficiency in vitro was evaluated via CCK-8 assay. The transmembrane transportation was evaluated via HPLC assay. The antitumor efficiency in vivo was assessed in C6 glioma intracranial implant rat model. The average diameter of Tf-NP-Dox was 100nm with approximate to 32 Tf molecules on the surface. DLC was 4.4%. CCK-8 assay demonstrated much stronger cytotoxicity of Tf-NP-Dox to C6 glioma cells compared to NP-Dox or Dox. HPLC assay showed that Tf-NP-Dox transported Dox into C6 cells with high efficiency. In vivo, Tf-NP-Dox could transport Dox into tumors compare to contralateral part, with tumor inhibitory ratio and survival higher than NP-Dox or Dox. Taken together, our results suggest that Tf-NP-Dox exhibits better therapeutic effects against glioma both in vitro and in vivo, and is a potential nano-scale drug delivery system for glioma chemotherapy.
基金：
This work was supported by the University Graduate
Students’ Scientific Research Innovative Program of Jiangsu
Province, China (CX10B-0502) and the National Science
Foundation of People’s Republic of China (81272799, 81271554).
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外文
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出版当年[2012]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学4 区药学4 区核医学
最新[2023]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学4 区药学4 区核医学
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出版当年[2011]版：
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3PHARMACOLOGY & PHARMACYQ3ONCOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3MEDICINE, RESEARCH & EXPERIMENTALQ3ONCOLOGYQ3PHARMACOLOGY & PHARMACY
