机构：[1]Neurosurgery Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China[2]Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou, 215123, China[3]Cardiovascular Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China[4]Radiology Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China[5]Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, 111 Ren,ai Road, Suzhou, 215123, China[6]Graduate University of Chinese Academy of Sciences, 19(A) Yuquan Road, Beijing, 100039, China[7]Institute of Chemistry, Chinese Academy of Sciences, Zhong Guan Cun Bei Yi Jie 2, Beijing, 100190, China
Transferrin (Tf), an iron-transporting serum glycoprotein that binds to receptors overexpressed at the surface of glioma cells, was chosen as the ligand to develop Tf-conjugated PEGylated nanoscaled graphene oxide (GO) for loading and glioma targeting delivery of anticancer drug doxorubicin (Dox) (Tf-PEG-GO-Dox). Tf-GO with lateral dimensions of 100-400 nm exhibited a Dox loading ratio up to 115.4%. Compared with Dox-loaded PEGylated GO (PEG-GO-Dox) and free Dox, Tf-PEG-GO-Dox displayed greater intracellular delivery efficiency and stronger cytotoxicity against C6 glioma cells. A competition test showed that Tf was essential to glioma targeting in vitro. The HPLC assay for Dox concentration in tumor tissue and contrapart tissue of the brain demonstrated that Tf-PEG-GO-Dox could deliver more Dox into tumor in vivo. The life span of tumor bearing rats after the administration of Tf-PEG-GO-Dox was extended significantly compared to the rats treated with saline, Dox, and PEG-GO-Dox In conclusion, we developed Tf-PEG-GO-Dox which exhibited significantly improved therapeutic efficacy for glioma both in vitro and in vivo.
基金：
This work was supported by University Graduate Students’
Scientific Research Innovative Program of Jiangsu Province,
China (CX10B-0502) and National Science Foundation of P.
R. China (51361130033, 81272799, 81271554, and 21073224).
第一作者机构：[1]Neurosurgery Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China
共同第一作者：
通讯作者：
通讯机构：[1]Neurosurgery Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, China[2]Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou, 215123, China
推荐引用方式(GB/T 7714)：
Guodong Liu,He Shen,Jinning Mao,et al.Transferrin Modified Graphene Oxide for Glioma-Targeted Drug Delivery: In Vitro and in Vivo Evaluations[J].ACS APPLIED MATERIALS & INTERFACES.2013,5(15):6909-14.doi:10.1021/am402128s.
APA：
Guodong Liu,He Shen,Jinning Mao,Liming Zhang,Zhen Jiang...&Zhijun Zhang.(2013).Transferrin Modified Graphene Oxide for Glioma-Targeted Drug Delivery: In Vitro and in Vivo Evaluations.ACS APPLIED MATERIALS & INTERFACES,5,(15)
MLA：
Guodong Liu,et al."Transferrin Modified Graphene Oxide for Glioma-Targeted Drug Delivery: In Vitro and in Vivo Evaluations".ACS APPLIED MATERIALS & INTERFACES 5..15(2013):6909-14
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