机构：[1]School of Materials Science and Engineering, Peking University, Beijing 100871, China[2]China-America Institute of Neuroscience and Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China首都医科大学宣武医院内科系统老年医学科科技平台中美神经科学研究所[3]Columbia University Department of Biomedical Engineering, New York, NY, USA[4]Shaanxi Key Laboratory of biomedical metallic materials, Northwest Institute for Nonferrous Metal Research, Xi’an, 710016, China[5]Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
To better mimic the inherent porous architecture of the natural bone, we fabricated graphene oxide/silk fibroin (GO/SF) scaffolds using a salting leaching method, followed by in situ reduction and reinforcement with ascorbic acid. SEM images revealed that the scaffolds exhibited an interconnectivity porous structure both on the surface and throughout the interior. We found that the pore size could be readily adjusted by varying the concentration of GO in the scaffolds. FTIR results demonstrated that the addition of GO didn't form chemical interaction between GO and SF, but it significantly increased the content of beta-sheet structure from 46.36 % to 51.12 % and improved the thermal stability of the scaffolds. Moreover, the reduced graphene oxide/silk fibroin (rGO/SF) scaffolds exhibited a superior capacity to promote the adhesion, proliferation, and mineral deposition of mouse mesenchymal stem cell (MSCs) when compared to pure SF scaffolds. Notably, the equilibrium Young's modulus of the scaffolds gradually increased from 5.5 +/- 0.5 KPa to 14.6 +/- 2.1 KPa with the increment of rGO content. Osteogenic differentiation with higher BSP (2. 84-fold) and RUNX2 (2.91-fold) expression level was also observed in 3 % rGO scaffold, suggesting a strong inclination towards bone formation. Based on these findings, the prepared rGO/SF scaffold in this study hold great potential as a viable option for bone tissue engineering.
基金：
National Natural Science Foundation of China [31070846, 82102220, 32101087]; China Scholarship Council; Research Funding on Translational Medicine from Beijing Municipal Science and Technology Commission [Z221100007422023]; Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support from Yangfan Project [YGLX202325]; Outstanding Young Talents Program of Capital Medical University [B2305]; Non-profit Central Research Institute Fund of Chinese Academy of Medical [2023-JKCS-09]; Shaanxi Qinchuangyuan Cited High-level Innovation and Entrepreneurial Talents Project [QCYRCXM-2022-156, QCYRCXM-2022-52]
第一作者机构：[1]School of Materials Science and Engineering, Peking University, Beijing 100871, China[2]China-America Institute of Neuroscience and Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
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推荐引用方式(GB/T 7714)：
Li Ming,Cao Xiaofeng,Zhang Hongkang,et al.Improved biocompatibility and osteogenesis of porous graphene oxide/silk fibroin scaffold for potential applications in bone tissue engineering[J].MATERIALS TODAY COMMUNICATIONS.2024,40:doi:10.1016/j.mtcomm.2024.109480.
APA：
Li, Ming,Cao, Xiaofeng,Zhang, Hongkang,Li, Ang,Zhou, Wenhao...&Zheng, Yufeng.(2024).Improved biocompatibility and osteogenesis of porous graphene oxide/silk fibroin scaffold for potential applications in bone tissue engineering.MATERIALS TODAY COMMUNICATIONS,40,
MLA：
Li, Ming,et al."Improved biocompatibility and osteogenesis of porous graphene oxide/silk fibroin scaffold for potential applications in bone tissue engineering".MATERIALS TODAY COMMUNICATIONS 40.(2024)
