机构：[1]Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou Jiangsu, P.R. China[2]Jiangsu Province Key Laboratory of Stem Cell Research, Medical College of Soochow University, Suzhou Jiangsu, P.R. China[3]Material Engineering Institute, Soochow University, Suzhou Jiangsu, P.R. China
Transplantation of olfactory ensheathing cells (OECs) is a potential treatment for spinal cord injury (SCI) However, this process lacks extracellular matrix guiding cell growth, tissue morphogenesis, and remodeling In order to solve this problem. we fabricated silk fibroin scaffolds (SFS) with different fiber diameters by electrospinning The behaviors of OECs on 300 and 1800 nm SFS were studied by analyzing cell morphological feature, distribution, and proliferation The results showed the 300 rim SFS with good potential to guide OECs growth Subsequently, the properties of 300 nm SFS were further investigated along with PLL With 300 rim SFS, the preservation of cell phenotype was confirmed by the presence of cell-specific markers. Including nerve growth factor receptor p75 and ghal fibrillary acidic protein And the migration behaviors of OECs were also observed by Leica AF6000 In addition, migration tracks, turning behavior, migration distances, migration speeds, and forward migration indices were calculated Furthermore, the expression of neurotrophic facto's was assayed at transcription and protein levels using RT-PCR and ELISA All these results indicated the diameter of the fiber played an important role in guiding cell adhesion, growth, and migration in vitro and the 300 nm SFS could be suitable to construct tissue-engineered scaffolds for SCI repair
基金：
This work was funded by health department scientific research projects of Jiangsu Province
(H200920), and Science & Research Innovation Plan of Postgraduates in Colleges and Universities of Jiangsu Province
(ZY320717), and supported by Jiangsu Province Key Laboraof Stem Cell Research
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2009]版：
大类|2 区生物
小类|2 区移植3 区细胞生物学
最新[2023]版：
大类|4 区医学
小类|4 区细胞与组织工程4 区医学：研究与实验4 区移植
JCR分区：
出版当年[2008]版：
Q1CELL BIOLOGYQ1TRANSPLANTATION
最新[2023]版：
Q2MEDICINE, RESEARCH & EXPERIMENTALQ2TRANSPLANTATIONQ3CELL & TISSUE ENGINEERING
第一作者机构：[1]Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou Jiangsu, P.R. China[*1]Department of Orthopedics, the Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou Jiangsu 215006, P.R. China.
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Orthopedics, the Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou Jiangsu 215006, P.R. China.
推荐引用方式(GB/T 7714)：
Yixin Shen,Yuqiang Qian,Huanxiang Zhang,et al.Guidance of Olfactory Ensheathing Cell Growth and Migration on Electrospun Silk Fibroin Scaffolds[J].CELL TRANSPLANTATION.2010,19(2):147-57.doi:10.3727/096368910X492616.
APA：
Yixin Shen,Yuqiang Qian,Huanxiang Zhang,Baoqi Zuo,Zhenfeng Lu...&Chunlei Zhou.(2010).Guidance of Olfactory Ensheathing Cell Growth and Migration on Electrospun Silk Fibroin Scaffolds.CELL TRANSPLANTATION,19,(2)
MLA：
Yixin Shen,et al."Guidance of Olfactory Ensheathing Cell Growth and Migration on Electrospun Silk Fibroin Scaffolds".CELL TRANSPLANTATION 19..2(2010):147-57
