机构：[a]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China[b]Department of Orthopaedics, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou 215021, China
The aim of this study was to determine the effects of ginsenoside Rg1 on the migration of olfactory ensheathing cells (OECs) in vitro, and its influence on the therapeutic efficacy of OECs transplanted in vivo for the treatment of spinal cord injury (SCI). Primary cultured and purified OECs (prepared from rats) were treated with ginsenoside Rg1. The wound healing test indicated that ginsenoside Rg1 promoted the migration of OECs. Real-time RT-PCR demonstrated that ginsenoside Rg1 upregulated the expression of migration-related factors of OECs, including matrix metalloproteinases-2 (MMP-2), MMP-9, and neural cell adhesion molecule 1 (NCAM1). Moreover, Western blot analysis indicated that ginsenoside Rg1 significantly promoted the migration of OECs via the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. An SCI rat model was induced in vivo using a revised Allen's method. The Basso, Beattie, and Bresnahan (BBB) scores and histological analysis demonstrated that OECs, which were treated with ginsenoside Rg1, exhibited significant improvement in SCI compared with both the control group and the OEC group. Thus, ginsenoside Rg1 may represent a novel treatment target for SCI.
基金：
This study was supported by the
National Natural Science Foundation of China (81330026,
30870642), Jiangsu Province Natural Science Foundation
(BK20141186).
第一作者机构：[a]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China
共同第一作者：
通讯作者：
通讯机构：[a]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China
推荐引用方式(GB/T 7714)：
Yin-Yao Tang ,Wei-Xiao Guo ,Zheng-Feng Lu,et al.Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury[J].BIOLOGICAL & PHARMACEUTICAL BULLETIN.2017,40(10):1630-1637.doi:10.1248/bpb.b16-00896.
APA：
Yin-Yao Tang,,Wei-Xiao Guo,,Zheng-Feng Lu,Mao-Hua Cheng,,Yi-Xin Shen,&Ying-Zi Zhang.(2017).Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury.BIOLOGICAL & PHARMACEUTICAL BULLETIN,40,(10)
MLA：
Yin-Yao Tang,,et al."Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury".BIOLOGICAL & PHARMACEUTICAL BULLETIN 40..10(2017):1630-1637
