机构：[1]Department of Materials Physics and Chemistry, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China[2]Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China血管外科首都医科大学宣武医院
A continuous electrospinning technique was applied to fabricate double layer tubular tissue engineering vascular graft (TEVG) scaffold. The luminal layer was made from poly(-caprolac-tone)(PCL) ultrafine fibers via common single axial electrospinning followed by the outer layer of core-shell structured nanofibers via coaxial electrospinning. For preparing the outer layernano-fibers, the PCL was electrospun into the shell and both bovine serum albumin (BSA) and tetrapeptide val-gal-pro-gly (VAPG) were encapsulated into the core. The core-shell structure in the outer layer fibers was observed by transmission electron microscope (TEM). The in vitro release tests exhibited the sustainable release behavior of BSA and VAPG so that they provided a better cell growth environment in the interior of tubular scaffold wall. The in vitro culture of smooth muscle cells (SMCs) demonstrated their potential to penetrate into the scaffold wall for the 3D cell culture. Subsequently, 3D cell coculture was conducted. First, SMCs were seeded on the luminal surface of the scaffold and cultured for 5 days, and then endothelial cells (ECs) were also seeded on the luminal surface and cocultured with SMCs for another 2 days. After stained with antibodies, 3D cell distribution on the scaffold was revealed by confocal laser scanning microscopy (CLSM) where ECs were mainly located on the luminal surface whereas SMCs penetrated into the surface and distributed inside the scaffold wall. This double layer tubular scaffold with 3D cell distribution showed the promise to develop it into a novel TEVG for clinical trials in the near future. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3863-3871, 2015.
基金：
“863” Project of Minister of Science and Technology of China; contract (grant number: 2012AA020507)
Beijing Institute of Technology; contract (grant number: 20120942005)
第一作者机构：[1]Department of Materials Physics and Chemistry, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
通讯作者：
通讯机构：[1]Department of Materials Physics and Chemistry, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China
推荐引用方式(GB/T 7714)：
Lin Ye ,Jie Cao ,Lamei Chen ,et al.The fabrication of double layer tubular vascular tissue engineering scaffold via coaxial electrospinning and its 3D cell coculture[J].JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A.2015,103(12):3863-3871.doi:10.1002/jbm.a.35531.
APA：
Lin Ye,,Jie Cao,,Lamei Chen,,Xue Geng,,Ai-Ying Zhang,...&Zeng-Guo Feng.(2015).The fabrication of double layer tubular vascular tissue engineering scaffold via coaxial electrospinning and its 3D cell coculture.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,103,(12)
MLA：
Lin Ye,,et al."The fabrication of double layer tubular vascular tissue engineering scaffold via coaxial electrospinning and its 3D cell coculture".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103..12(2015):3863-3871
