机构：[1]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[2]Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China[3]Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[4]Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[5]Department of Vascular Surgery, Beijing Anzhen Hospital of Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing 100029, China临床科室血管科首都医科大学附属安贞医院[6]Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[7]Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[8]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA[9]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA
Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity of hPSC-VSMCs with current cell culture technologies remains very challenging. Here, we report a scalable method for manufacturing hPSC-VSMCs in alginate hydrogel microtubes (i.e., AlgTubes), which protect cells from hydrodynamic stresses and limit cell mass to <400 mu m to ensure efficient mass transport. The tubes provide cells a friendly microenvironment, leading to extremely high culture efficiency. We have shown that hPSC-VSMCs can be generated in 10 days with high viability, high purity, and high yield (similar to 5.0 x 10(8) cells/mL). Phenotype and gene expression showed that VSMCs made in AlgTubes and VSMCs made in 2D cultures were similar overall. However, AlgTube-VSMCs had higher expression of genes related to vasculature development and angiogenesis, and 2D-VSMCs had higher expression of genes related to cell death and biosynthetic processes.
基金：
UNL-UNMC Science Engineering and Medicine (SEM) initiative grant
第一作者机构：[1]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
共同第一作者：
通讯作者：
通讯机构：[1]Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[4]Biomedical Engineering Program, University of Nebraska-Lincoln, Lincoln, NE 68588, USA[8]Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA[9]Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA
推荐引用方式(GB/T 7714)：
Haishuang Lin ,Xuefeng Qiu ,Qian Du ,et al.Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells[J].STEM CELL REPORTS.2019,12(1):84-97.doi:10.1016/j.stemcr.2018.11.009.
APA：
Haishuang Lin,,Xuefeng Qiu,,Qian Du,,Qiang Li,,Ou Wang,...&Yuguo Lei.(2019).Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells.STEM CELL REPORTS,12,(1)
MLA：
Haishuang Lin,,et al."Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells".STEM CELL REPORTS 12..1(2019):84-97
医学